apolipoprotein E/apoE Double Nickase Plasmid (h): sc-400860-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apolipoprotein E/apoE Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- apolipoprotein E/apoE Double Nickase Plasmid (h) and apolipoprotein E/apoE Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APOE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: apolipoprotein E/apoE Antibody (F-9): sc-390925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apolipoprotein E/apoE Double Nickase Plasmid (h) | sc-400860-NIC | 20 µg | $410.00 | |||
apolipoprotein E/apoE Double Nickase Plasmid (h2) | sc-400860-NIC-2 | 20 µg | $410.00 |
Human APOE encodes apolipoprotein E (apoE), a secreted lipoprotein component that mediates cholesterol and triglyceride transport by binding receptors such as LDLR and LRP1 and coordinating lipoprotein clearance and redistribution. ApoE influences lipid homeostasis, membrane remodeling, and receptor-driven endocytosis, linking it to pathways that regulate cellular cholesterol efflux, lipoprotein particle metabolism, and inflammatory signaling in tissue-resident macrophages and glia. Genetic variation and altered expression of APOE are strongly associated with dysregulated lipid handling and neuroinflammatory processes relevant to neurodegeneration, as well as atherosclerosis-related biology. Accordingly, APOE is frequently studied in systems modeling lipid trafficking, synaptic maintenance, and immune-metabolic crosstalk.
apolipoprotein E/apoE Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APOE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APOE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APOE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APOE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.