APC Double Nickase Plasmid (m): sc-419146-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APC Double Nickase Plasmid (m) and APC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Apc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: APC Antibody (F-3): sc-9998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC Double Nickase Plasmid (m) | sc-419146-NIC | 20 µg | $410.00 | |||
APC Double Nickase Plasmid (m2) | sc-419146-NIC-2 | 20 µg | $410.00 |
Mouse Apc encodes the APC tumor suppressor, a multifunctional scaffold that restrains canonical Wnt/β-catenin signaling by promoting β-catenin turnover through the destruction complex with AXIN, GSK3β, and CK1. Beyond Wnt regulation, APC contributes to cytoskeletal organization, cell polarity, and microtubule dynamics, shaping processes such as mitotic progression and directional migration. Disruption of APC-dependent signaling and cytoskeletal control is strongly linked to aberrant proliferation and intestinal homeostasis defects in model systems, making Apc a central node for studying pathways that couple transcriptional programs to tissue architecture. These properties position Apc as a key genetic driver for interrogating Wnt pathway outputs, epithelial differentiation, and genotype–phenotype relationships in mouse cells.
APC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Apc locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Apc. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Apc function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Apc-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.