APC Double Nickase Plasmid (h): sc-400374-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APC Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APC Double Nickase Plasmid (h) and APC Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: APC Antibody (F-3): sc-9998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC Double Nickase Plasmid (h) | sc-400374-NIC | 20 µg | $410.00 | |||
APC Double Nickase Plasmid (h2) | sc-400374-NIC-2 | 20 µg | $410.00 |
APC encodes adenomatous polyposis coli, a multifunctional tumor suppressor that scaffolds the β-catenin destruction complex and thereby regulates canonical Wnt signaling and transcriptional programs controlling proliferation and differentiation. Beyond Wnt pathway control, APC coordinates cytoskeletal dynamics, cell polarity, and chromosome segregation through interactions with microtubules, actin-associated factors, and kinetochore components. Disruption of APC function is strongly linked to aberrant β-catenin stabilization, altered cell fate decisions, and genomic instability, making APC a central node for studying intestinal epithelial homeostasis and oncogenic signaling mechanisms. Human APC is therefore widely used to interrogate pathway rewiring in models of colorectal cancer and related Wnt-driven phenotypes.
APC Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.