Annexin V Binding Buffer A buffer used with Annexin V reagents that is used for flow cytometry and immunofluorescence applications.

Annexin V Binding Buffer

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应用; A buffer used with Annexin V reagents that is used for flow cytometry and immunofluorescence applications
* 参考分析证明 大量特定数据 (包括水 含量).
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Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml. The binding buffer contains an optimal concentration of calcium which is required for Annexin V binding to phosphotidylserine (PS) on the cell surface. Annexin V is a calcium dependent phospholipid binding protein that preferentially binds to negatively charged phospholipids including PS. Cells progressing through apoptosis can be monitored according to their Annexin V and propidium iodide staining pattern. Early apoptotic cells will bind Annexin V but are not sensitive to intracellular staining with propidium iodide (PI). As cells progress through apoptosis the integrity of the plasma membrane is lost, allowing PI to penetrate and label the cells with a strong yellow-red fluorescence.


参考文献

1. Vermes, I. 1995. J. Immunol. Methods. 184(1): 39-51. PMID: 7622868

用法 :
Annexin V Binding Buffer is a optimal formula containing 2.5 mM Calcium Chloride in HEPES buffered saline.
Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml.
规划 :
2.5 mM Calcium Chloride in HEPES buffered saline
物理状态 :
Liquid
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。

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PMID: # 27070082  Corcelle-Termeau, E. et al. 2016. Autophagy. 12: 833-49.

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