Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Ankyrin G Antibody (463) - Western Blotting - Image 109666
Ankyrin G 抗体 (463): sc-12719. SK-N-MC (A), H4 (B), SK-N-SH (C), SH-SY5Y (D), IMR-32 (E) 和 TE671 (F) 全细胞裂解液中 Ankyrin G 表达的 Western 印迹分析.
Ankyrin G 抗体 (463): sc-12719. SK-N-MC (A), H4 (B), SK-N-SH (C), SH-SY5Y (D), IMR-32 (E) 和 TE671 (F) 全细胞裂解液中 Ankyrin G 表达的 Western 印迹分析.

Ankyrin G 抗体 (463): sc-12719

4.3(12)
寫評論提問

说明书
  • Ankyrin G 抗体 463 是小鼠单克隆 IgG1 κ，Ankyrin G抗体, 在58篇文献中引用，规格为200 µg/ml
  • 针对源自Ankyrin G human的spectrin结合域的合成肽
  • Ankyrin G 抗体 (463) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Ankyrin G; 也和以下物种反应，包括： canine
  • 抗Ankyrin G抗体（463）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ankyrin G (463): sc-12719的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Ankyrin G 抗体 (463)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Ankyrin 抗体 包括 Ankyrin-1, Ankyrin B和Ankyrin G

Ankyrin G 抗体（463）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的 Ankyrin G 蛋白。抗Ankyrin G抗体（463）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。锚蛋白G在维持神经元细胞完整性方面发挥着关键作用，它通过锚定钠通道在Ranvier节点和轴突起始段，这对于神经元中动作电位正常传播至关重要。这种定位确保了有效、快速的电信号传输，这是正常神经系统功能的基础。锚蛋白G参与细胞骨架组织和细胞形状的维持，进一步凸显其在细胞结构中的重要性。Ankyrin G蛋白具有组织特异性mRNA处理功能，在神经组织中形成两种主要同工型，而在其他组织中则形成较小的同工型，包括肺、肾、脾、肝和心脏。这种表达多样性表明Ankyrin G蛋白在神经元和非神经元环境中的多功能性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ankyrin G 抗体 (463) 参考文献:

  1. 轴突鞘氨醇在髓鞘前轴突存活和电压门控钠通道定域中的生理作用。  |  Bennett, V. and Lambert, S. 1999. J Neurocytol. 28: 303-18. PMID: 10739573
  2. 大鼠下肺泡神经损伤后三叉神经节中钠离子通道 SNS/PN3 和ankyrin(G) mRNA 的表达。  |  Bongenhielm, U., et al. 2000. Exp Neurol. 164: 384-95. PMID: 10915577
  3. 锚蛋白，一种参与多种细胞通路的多功能蛋白质。  |  Hryniewicz-Jankowska, A., et al. 2002. Folia Histochem Cytobiol. 40: 239-49. PMID: 12219834
  4. 疼痛性神经瘤：结构性跨膜蛋白锚蛋白 G 的潜在作用。  |  Kretschmer, T., et al. 2002. J Neurosurg. 97: 1424-31. PMID: 12507143
  5. 人支气管上皮细胞的侧膜生物生成需要 190-kDa 锚蛋白-G。  |  Kizhatil, K. and Bennett, V. 2004. J Biol Chem. 279: 16706-14. PMID: 14757759
  6. 炔蛋白的同工酶特异性。ankyrin-b分歧调控结构域中的两性α-螺旋与分子辅助伴侣Hdj1/Hsp40相互作用。  |  Mohler, PJ., et al. 2004. J Biol Chem. 279: 25798-804. PMID: 15075330
  7. 与疼痛相关的丛状神经纤维瘤和神经瘤中的锚蛋白 G 升高。  |  Kretschmer, T., et al. 2004. J Clin Neurosci. 11: 886-9. PMID: 15519868
  8. 导致 Brugada 综合征的 Nav1.5 E1053K 突变阻断了与 ankyrin-G 的结合以及 Nav1.5 在心肌细胞表面的表达。  |  Mohler, PJ., et al. 2004. Proc Natl Acad Sci U S A. 101: 17533-8. PMID: 15579534
  9. Ankyrin-G在神经精神疾病中的作用和机制。  |  Yoon, S., et al. 2022. Exp Mol Med. 54: 867-877. PMID: 35794211
  10. E-cadherin和Ankyrin-G之间的动态相互作用介导上皮细胞极性的维持。  |  Kong, C., et al. 2023. Nat Commun. 14: 6860. PMID: 37891324
  11. AnkyrinG.一种新的ankyrin基因，其神经特异性同工形式定域于轴突的节面和Ranvier节面。  |  Kordeli, E., et al. 1995. J Biol Chem. 270: 2352-9. PMID: 7836469
  12. 将 480/270-kD ankyrin G 限制在轴突近端需要多个 ankyrin G 特异性结构域。  |  Zhang, X. and Bennett, V. 1998. J Cell Biol. 142: 1571-81. PMID: 9744885

订购信息

产品名称产品编号规格价格数量收藏夹

Ankyrin G 抗体 (463)

sc-12719
200 µg/ml
$316.00

Ankyrin G 抗体 (463) AC

sc-12719 AC
500 µg/ml, 25% agarose
$416.00

Ankyrin G 抗体 (463) HRP

sc-12719 HRP
200 µg/ml
$316.00

Ankyrin G 抗体 (463) FITC

sc-12719 FITC
200 µg/ml
$330.00

Ankyrin G 抗体 (463) PE

sc-12719 PE
200 µg/ml
$343.00

Ankyrin G 抗体 (463) Alexa Fluor® 488

sc-12719 AF488
200 µg/ml
$357.00

Ankyrin G 抗体 (463) Alexa Fluor® 546

sc-12719 AF546
200 µg/ml
$357.00

Ankyrin G 抗体 (463) Alexa Fluor® 594

sc-12719 AF594
200 µg/ml
$357.00

Ankyrin G 抗体 (463) Alexa Fluor® 647

sc-12719 AF647
200 µg/ml
$357.00

Ankyrin G 抗体 (463) Alexa Fluor® 680

sc-12719 AF680
200 µg/ml
$357.00

Ankyrin G 抗体 (463) Alexa Fluor® 790

sc-12719 AF790
200 µg/ml
$357.00

I would like to try this out in canine samples for western blot Ankyrin G HRP is there any way to receive a test sample

Asked by: charlotte 280
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-12719, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for Ankyrin G available conjugated to agarose (sc-12719 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-12719 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-12719 PE), fluorescein (sc-12719 FITC), Alexa Fluor® 488 (sc-12719 AF488) or Alexa Fluor® 647 (sc-12719 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 13
Date published: 2017-01-04
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_12719, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from 很不错的产品用过santa的抗体，值得信赖.这次又要用新抗体了，还是想选择santade,并且查阅文献，大家用的也都是santa的抗体
Date published: 2018-11-20
Rated 4 out of 5 by from Works for well for immunocytochemistry in neurons!I bought this antibody for immunocytochemistry in murine cortical neurons. I used it at 1/50-1/100 and was very happy with the specific staining pattern.
Date published: 2018-05-02
Rated 5 out of 5 by from Superb!!Great western very specific ihc staining; just a great product
Date published: 2017-01-03
Rated 4 out of 5 by from Works wellTried Ank-G on mouse forebrain neuronal cultures. 3 day cultured neurons showed clear staining of the axon initial segment.
Date published: 2016-12-24
Rated 5 out of 5 by from A great Ankyrin G antibody!This antibody is capable of detecting multiple splice variants in a wide range of hosts. It will provide good WB data and even better staining. It's been cited more than 30 times!
Date published: 2016-12-22
Rated 4 out of 5 by from Published IHC(Pfa) data for use in mousePublished IHC(Pfa) data for use in mouse telencephalons. -SCBT Publication Review
Date published: 2015-06-04
Rated 4 out of 5 by from Published data for IF(pfa) in rhesus macaquePublished data for IF(pfa) in rhesus macaque monkey (Macaca mulatta) brain. -SCBT Publication Review
Date published: 2015-05-01
Rated 4 out of 5 by from Large amount of signal seen in WB in CacoLarge amount of signal seen in WB in Caco-2 whole cell lysate. -SCBT QC
Date published: 2015-04-16
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_12719, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
Ankyrin G 抗体 (463) is rated 4.3 out of 5 by 12.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_12719, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。