ANKRD13D Double Nickase Plasmid (h): sc-415493-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKRD13D Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ANKRD13D Double Nickase Plasmid (h) and ANKRD13D Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANKRD13D. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRD13D Double Nickase Plasmid (h) | sc-415493-NIC | 20 µg | $410.00 | |||
ANKRD13D Double Nickase Plasmid (h2) | sc-415493-NIC-2 | 20 µg | $410.00 |
ANKRD13D encodes an ankyrin repeat domain–containing protein implicated in protein–protein interactions that coordinate endocytic trafficking and membrane-associated quality control. Members of the ANKRD13 family have been linked to ubiquitin-dependent sorting events and regulation of receptor turnover, suggesting a role in coupling ubiquitination signals to vesicular transport pathways. Through these processes, ANKRD13D is relevant to studies of proteostasis, endosome dynamics, and signaling attenuation at the plasma membrane. Dysregulation of trafficking and ubiquitin-mediated sorting is frequently associated with altered growth factor signaling and cellular stress responses, making ANKRD13D a useful target for mechanistic investigation in disease-relevant models.
ANKRD13D Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANKRD13D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANKRD13D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANKRD13D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANKRD13D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.