Date published: 2025-9-6
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Amylin Antibody (E-5) - Immunohistochemistry - Image 286537
淀粉样蛋白抗体 (E-5): sc-377530. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的细胞质和膜染色.
淀粉样蛋白抗体 (E-5): sc-377530. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的细胞质和膜染色.

Amylin 抗体 (E-5): sc-377530

4.6(5)
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说明书
  • Amylin 抗体 E-5 是小鼠单克隆 IgG2a κ，Amylin抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列40-89位于human物种的Amylin的C-terminus
  • Amylin 抗体 (E-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Amylin precursor and active peptide
  • 抗Amylin抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Amylin (E-5): sc-377530的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 是Amylin 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Amylin 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Amylin抗体（E-5）是一种IgG2a κ小鼠单克隆Amylin抗体（也称为胰岛淀粉样多肽抗体、DAP抗体、糖尿病相关肽抗体、胰岛素瘤淀粉样肽抗体、IAP抗体、胰岛淀粉样多肽（糖尿病相关肽或Amylin）抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的Amylin蛋白。Amylin抗体（E-5）既有非共轭形式的抗Amylin抗体，也有多种共轭形式的抗Amylin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。肾上腺髓质素（AM）、α-和β-降钙素基因相关肽（CGRP）、降钙素（CT）和Amylin是具有重叠生物作用的同源多肽，如血管舒张和抑制骨吸收。Amylin（胰岛/胰岛素瘤淀粉样多肽或IAPP）是从胰腺淀粉样蛋白中分离出的37个氨基酸的单体多肽。该蛋白是2型糖尿病患者富含淀粉样蛋白的胰腺提取物的主要成分。Amylin在2和7位有半胱氨酸残基，这是所有已知的降钙素基因相关肽中都有的特征，与CGRP II的氨基酸序列同源性为46%。在几种哺乳动物的正常β细胞中，Amylin可能在胰岛功能中发挥重要作用。编码Amylin的基因位于人类染色体12p12.3-p12.1上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Amylin 抗体 (E-5) 参考文献:

  1. 肾上腺髓质素肽家族的 G 蛋白偶联受体与附属受体活性修饰蛋白（RAMP）之间的功能性相互作用。  |  Born, W., et al. 2002. Microsc Res Tech. 57: 14-22. PMID: 11921352
  2. 人类胰岛中的淀粉酶。  |  Tomita, T. 2003. Pathology. 35: 34-6. PMID: 12701681
  3. 纤溶淀粉样蛋白通过与 Caspase cascade 和 JNK1 的关联激活唤起胰岛β细胞凋亡。  |  Zhang, S., et al. 2003. J Biol Chem. 278: 52810-9. PMID: 14532296
  4. 人类淀粉样蛋白寡聚体的生长和纤维的伸长是淀粉样蛋白形成过程中两个截然不同的阶段。  |  Green, JD., et al. 2004. J Biol Chem. 279: 12206-12. PMID: 14704152
  5. 外周淀粉样蛋白可激活大鼠体内表达降钙素受体 a/b 亚型和受体活性修饰蛋白的环状器官。  |  Barth, SW., et al. 2004. Brain Res. 997: 97-102. PMID: 14715154
  6. 淀粉酶抑制骨吸收，而降钙素受体则控制体内骨形成。  |  Dacquin, R., et al. 2004. J Cell Biol. 164: 509-14. PMID: 14970190
  7. 平行叠层β结构作为人淀粉样蛋白淀粉样纤维的模型。  |  Kajava, AV., et al. 2005. J Mol Biol. 348: 247-52. PMID: 15811365
  8. 缬沙坦抑制淀粉样蛋白诱导的足细胞损伤。  |  Huang, F., et al. 2016. Microvasc Res. 106: 101-9. PMID: 27102209
  9. 从 2 型糖尿病患者富含淀粉样蛋白的胰腺中纯化一种多肽并确定其特征。  |  Cooper, GJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 8628-32. PMID: 3317417
  10. CGRP以外偏头痛治疗的未来目标。  |  Al-Hassany, L., et al. 2023. J Headache Pain. 24: 76. PMID: 37370051
  11. 皮肤毛细血管淀粉样蛋白沉积类似于大鼠脑淀粉样蛋白血管病变。  |  Das, S., et al. 2023. J Stroke Cerebrovasc Dis. 32: 107300. PMID: 37572602
  12. 人类降钙素/CGRP/IAPP 基因家族成员的高分辨率染色体定位。  |  Hoovers, JM., et al. 1993. Genomics. 15: 525-9. PMID: 8468047

订购信息

产品名称产品编号规格价格数量收藏夹

Amylin 抗体 (E-5)

sc-377530
200 µg/ml
$316.00

Amylin (E-5): m-IgG2a BP-HRP 套装

sc-545954
200 µg Ab; 10 µg BP
$354.00

Amylin 抗体 (E-5) AC

sc-377530 AC
500 µg/ml, 25% agarose
$416.00

Amylin 抗体 (E-5) HRP

sc-377530 HRP
200 µg/ml
$316.00

Amylin 抗体 (E-5) FITC

sc-377530 FITC
200 µg/ml
$330.00

Amylin 抗体 (E-5) PE

sc-377530 PE
200 µg/ml
$343.00

Amylin 抗体 (E-5) Alexa Fluor® 488

sc-377530 AF488
200 µg/ml
$357.00

Amylin 抗体 (E-5) Alexa Fluor® 546

sc-377530 AF546
200 µg/ml
$357.00

Amylin 抗体 (E-5) Alexa Fluor® 594

sc-377530 AF594
200 µg/ml
$357.00

Amylin 抗体 (E-5) Alexa Fluor® 647

sc-377530 AF647
200 µg/ml
$357.00

Amylin 抗体 (E-5) Alexa Fluor® 680

sc-377530 AF680
200 µg/ml
$357.00

Amylin 抗体 (E-5) Alexa Fluor® 790

sc-377530 AF790
200 µg/ml
$357.00

Hi, do you have the AA sequence of this antibody?

Asked by: Pawel
Thank you for your question. The sequence is proprietary but here is a link to more information on the protein. https://www.uniprot.org/uniprotkb/P10997/entry
Answered by: BlakeJ
Date published: 2024-10-24

What are the components of the buffer in which #sc-377530 is delivered?

Asked by: elaj
Thank you for your question. This antibody is supplied as 200 µg in 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2024-10-18

What do you mean by the antibody detecting "Amylin precursor and active peptide"? Does this refer to monomeric or oligomeric amylin?

Asked by: KaraZ
Thank you for your question. This monoclonal antibody is raised against amino acids 40-89 of the full length human protein, which, according to Uniprot is processed into a Islet amyloid polypeptide, corresponding to amino acids 34-70. Therefore, we expect the antibody to recognize both precursor and active processed forms of Amylin. https://www.uniprot.org/uniprot/P10997#ptm_processing
Answered by: Technical Support
Date published: 2022-11-30

How specific is the antibody for human amylin? Is there any cross reactivity for amylin of other species (especially rat)?

Asked by: Susan1
Thank you for your question. We have not yet completed testing of this antibody, Amylin (E-5): sc-377530, for reactivity in other species including mouse and rat. The antibody is raised against an antigen sequence derived from human Amylin, however that sequence does share ~ 70% sequence identity with the corresponding protein in mouse and rat. The exact epitope is not known for this antibody, but there is potential for cross-reactivity with these other species.
Answered by: Technical Support
Date published: 2017-11-02

Is Amylin Antibody (E-5): sc-377530 appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenni
Thank you for your question. Yes, Amylin Antibody (E-5): sc-377530 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Service 11
Date published: 2017-01-09

Hi, l would like to use your Amylin antibody E-5 and H-50 as bioreceptors in my Biacore assay, to detect Amylin as analyte based on antigen-antibody binding. Could you please send me the links of Amylin that I should I buy? Thank you very much in advance!

Asked by: Evelyn
Thank you for your question. We have not tested any of our antibodies using the Biacore assay and so we are unable to offer you any specific support using the antibodies in this system. We do not currently sell any Amylin protein or peptide that could be used as a synthetic antigen with our Amylin antibodies.
Answered by: Technical Support
Date published: 2016-10-06
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Rated 5 out of 5 by from Good antibodyGood, published Amylin antibody for human detection. Strong signal observed in WB and staining, and is also recommended for IP and ELISA.
Date published: 2017-01-20
Rated 5 out of 5 by from Superb productOutstanding IHC staining data; also reacts well for WB
Date published: 2017-01-09
Rated 4 out of 5 by from Worked well in our WBWorked well in our WB.
Date published: 2015-08-05
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-10
Rated 4 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected whole cell lysates. -SCBT QC
Date published: 2015-02-05
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Amylin 抗体 (E-5) is rated 4.6 out of 5 by 5.
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