AMPKβ2 Double Nickase Plasmid (h): sc-403537-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMPKβ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AMPKβ2 Double Nickase Plasmid (h) and AMPKβ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRKAB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AMPKβ2 Antibody (C-7): sc-376752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMPKβ2 Double Nickase Plasmid (h) | sc-403537-NIC | 20 µg | $410.00 | |||
AMPKβ2 Double Nickase Plasmid (h2) | sc-403537-NIC-2 | 20 µg | $410.00 |
PRKAB2 encodes the β2 regulatory subunit of AMP-activated protein kinase (AMPK), a central energy sensor that coordinates cellular responses to nutrient availability and energetic stress. AMPKβ2 helps scaffold the catalytic α and regulatory γ subunits and contributes to complex stability, subcellular localization, and substrate selection, thereby influencing pathways that govern glucose uptake, fatty acid oxidation, autophagy, and inhibition of anabolic signaling through mTORC1. Through these functions, AMPKβ2 participates in maintaining metabolic homeostasis across tissues and shapes adaptive programs during hypoxia, mitochondrial stress, and exercise-like signaling. Dysregulation of AMPK signaling, including altered PRKAB2-associated activity, has been linked to metabolic disease phenotypes and is frequently studied in the context of cancer cell metabolism and stress tolerance.
AMPKβ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRKAB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRKAB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRKAB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRKAB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.