Date published: 2025-9-9

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ALX1 抗体 (96K): sc-130416

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说明书
  • ALX1 抗体 96K 是小鼠单克隆 IgG2a κ,ALX1抗体, 在1篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组ALX1
  • 推荐用于 mouse, rat 和 human 来源的ALX1 WB, IP 和 IF检测
  • 关于如何获取ALX1 (96K): sc-130416免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是ALX1 Antibody (96K) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ALX1 Antibody (96K) 打包提供(请参阅下面的订购信息)。

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関連項目

ALX1抗体(96K)是一种IgG2a κ小鼠单克隆ALX1抗体(也称为ALX1抗体),可通过WB、IP和IF检测小鼠、大鼠和人源的ALX1蛋白。ALX1抗体(96K)有非偶联的抗ALX1抗体形式可供选择。ALX同源异型盒蛋白是配对同源异型盒家族的成员,在胚胎阶段的细胞发育和模式形成中发挥作用。ALX1(ALX同源异型盒蛋白1),也称为CART-1(软骨同源蛋白-1),是一种特异于宫颈和软骨组织的326个氨基酸的蛋白质。除了具有同源异型盒结构域外,ALX1还包含一个OAR结构域,该结构域被认为对DNA结合或蛋白质-蛋白质相互作用和反式激活很重要。ALX1最初是从大鼠软骨肉瘤肿瘤细胞系中鉴定出来的,是一种同源异型盒转录因子,可调控下游靶基因,并已具体显示在体内作为大鼠催乳素的转录阻遏物。纯合子ALX1缺陷小鼠出生时患有无颅畸形和中脑无裂畸形,这表明ALX1在神经管发育中发挥作用。还有人认为ALX1与ALX4在肢体发育和颅面骨形成中起协同作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ALX1 抗体 (96K) 参考文献:

  1. 同源结构域蛋白Cart1的OAR/无芳香结构域在体内具有减弱作用。  |  Brouwer, A., et al. 2003. Mech Dev. 120: 241-52. PMID: 12559496
  2. P300/CBP 通过对邻近同源染色体的保守赖氨酸残基进行乙酰化,成为软骨同源蛋白-1(Cart1)(成对类同源蛋白)的辅助激活剂。  |  Iioka, T., et al. 2003. J Bone Miner Res. 18: 1419-29. PMID: 12929931
  3. 软骨同源蛋白 1,一种在软骨细胞中选择性表达的同源蛋白。  |  Zhao, GQ., et al. 1993. Proc Natl Acad Sci U S A. 90: 8633-7. PMID: 7690966
  4. 产前叶酸治疗可抑制Cart1同源基因突变小鼠的尖头畸形和无脑畸形。  |  Zhao, Q., et al. 1996. Nat Genet. 13: 275-83. PMID: 8673125
  5. 阐明同源蛋白 Cart-1 在 C3H10T1/2 微体细胞体外软骨形成过程中的功能。  |  Atkinson, BL., et al. 1996. Ann N Y Acad Sci. 785: 206-8. PMID: 8702130
  6. 人类 Cart-1:软骨特异性同源基因组 cDNA 的结构组织, 染色体定域和功能分析。  |  Gordon, DF., et al. 1996. DNA Cell Biol. 15: 531-41. PMID: 8756334
  7. 人类 CART1 是一种成对的同源结构域蛋白,通过对偶结合位点激活转录。  |  Cai, RL. 1998. Biochem Biophys Res Commun. 250: 305-11. PMID: 9753625
  8. Alx4 和 Cart1 之间的物理和遗传相互作用  |  Qu, S., et al. 1999. Development. 126: 359-69. PMID: 9847249

订购信息

产品名称产品编号规格价格数量收藏夹

ALX1 抗体 (96K)

sc-130416
100 µg/ml
$333.00

ALX1 (96K): m-IgG Fc BP-HRP 套装

sc-537332
100 µg Ab; 10 µg BP
$354.00

ALX1 (96K): m-IgGκ BP-HRP 套装

sc-534573
100 µg Ab; 40 µg BP
$354.00

ALX1 (96K): m-IgG2a BP-HRP 套装

sc-547982
100 µg Ab; 10 µg BP
$354.00

Is there any publication of ALX1 (96K): sc-130416?

Asked by: chell
Thank you very much for the question! We currently do not yet list any product citation for this antibody clone. However our labs reported excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells and we got a customer review saying' this antibody showed excellent cytoplasmic staining of neurons.' We of course provide all antibodies with a 1 year product warranty. This antibody is recommended by us for use in mouse, rat und human Samples in WB, IP and IF. If you have any further questions, please contact our Technical Service team. You can find the corresponding contact information, by clicking the following link: https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support Europe
Date published: 2017-11-09

I am using ALX1 (96K): sc-130416 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great choice for Western BlotThis antibody detects strong bands at the expected molecular weight in SH-SY5Y, SK-BR-3, HepG2, MCF-7, and SW480 nuclear extracts. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Excellent cytoplasmic staining of neuronsThis antibody showed excellent cytoplasmic staining of neurons.
Date published: 2017-02-14
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear stainingExceptional immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-16
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ALX1 抗体 (96K) is rated 5.0 out of 5 by 4.
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