Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α1a Tubulin CRISPR/Cas9 KO Plasmid (h): sc-400022
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α1a Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the α1a Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: α1a Tubulin Antibody (7-RY28): sc-134237
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α1a Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-400022 | 20 µg | $397.00 |
Overview
TUBA1A encodes α1a tubulin, a core α-tubulin isotype that heterodimerizes with β-tubulin to assemble microtubules, supporting cytoskeletal architecture, intracellular transport, and mitotic spindle function. Microtubule dynamics governed by α/β-tubulin composition and associated proteins coordinate processes such as neuronal migration, axon guidance, and vesicle trafficking. TUBA1A is enriched in the developing nervous system, and disruptions in microtubule polymerization or stability can perturb cortical development programs. Pathogenic variation in TUBA1A is linked to tubulinopathies and neurodevelopmental disorders characterized by malformations of cortical development and impaired neuronal connectivity.
α1a Tubulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUBA1A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUBA1A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUBA1A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish α1a Tubulin protein expression.
This CRISPR knockout system enables efficient generation of TUBA1A-deficient cell models for investigation of α1a Tubulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUBA1A exon(s) critical for α1a Tubulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUBA1A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by α1a Tubulin CRISPR/Cas9 KO Plasmid (h) and α1a Tubulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUBA1A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by α1a Tubulin HDR Plasmid (h) and α1a Tubulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUBA1A homology arms to support homology-directed repair at defined TUBA1A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.