Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aldolase B CRISPR/Cas9 KO Plasmid (m): sc-432929
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldolase B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Aldolase B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldolase B Antibody (C-11): sc-393278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldolase B CRISPR/Cas9 KO Plasmid (m) | sc-432929 | 20 µg | $397.00 |
Overview
Aldob encodes aldolase B, a glycolytic and fructolytic enzyme that cleaves fructose-1,6-bisphosphate and fructose-1-phosphate to generate triose phosphates, linking carbohydrate breakdown to downstream energy production and biosynthetic flux. In mouse, aldolase B activity is most prominent in hepatocytes and proximal renal tubule cells, supporting hepatic glucose homeostasis, fructose metabolism, and metabolic adaptation during fasting and feeding cycles. Disruption of ALDOB function is classically associated with impaired fructose handling and broader disturbances in glycolysis/gluconeogenesis balance, making it relevant for studying metabolic stress responses and hepatocyte physiology. Aldob also provides a tractable node for interrogating pathway crosstalk between glycolysis, gluconeogenesis, and fructose-driven signaling in liver-centered disease models.
Aldolase B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aldob gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aldob together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aldob open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldolase B protein expression.
This CRISPR knockout system enables efficient generation of Aldob-deficient cell models for investigation of Aldolase B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Aldob exon(s) critical for Aldolase B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Aldob genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Aldolase B CRISPR/Cas9 KO Plasmid (m) and Aldolase B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aldob locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Aldolase B HDR Plasmid (m) and Aldolase B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aldob homology arms to support homology-directed repair at defined Aldob target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.