Date published: 2025-9-7

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AK2 抗体 (F-2): sc-374095

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说明书
  • AK2 抗体 F-2 是小鼠单克隆 IgG1 κ,AK2抗体, 在5篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列31-95位于human物种的AK2的N-terminus附近
  • AK2 抗体 (F-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 AK2
  • 抗AK2抗体(F-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AK2 (F-2): sc-374095免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 AK2 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与AK2 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

AK2抗体(F-2)是一种IgG1 κ小鼠单克隆AK2抗体(也称为AK2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的AK2蛋白。AK2抗体(F-2)既可以是非偶联的抗AK2抗体形式,也可以是多种偶联形式的抗AK2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。腺苷酸激酶1-5(称为AK1-5)是一组酶,调节细胞内腺嘌呤核苷酸的磷酸化状态,后者是活细胞中的主要高能磷酸携带分子。腺苷酸激酶通过催化腺嘌呤核苷酸(AMP、ADP、ATP)之间的磷酸转移来影响代谢信号,包括基因表达、离子通道活性和蛋白激酶介导的信号传导。已发现,导致红细胞中AK缺陷的遗传性突变与溶血性贫血有关。AK2存在于肝和心脏组织的线粒体中,是唯一定位于线粒体膜间隙的AK。在凋亡细胞中,AK2是唯一与细胞色素c同时转移到细胞质中的AK,这表明在凋亡的早期阶段,只有膜间蛋白从线粒体释放。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AK2 抗体 (F-2) 参考文献:

  1. 细胞凋亡过程中线粒体膜间隙释放腺苷酸激酶 2  |  Köhler, C., et al. 1999. FEBS Lett. 447: 10-2. PMID: 10218571
  2. 腺苷酸激酶磷酸转移将细胞能量信号传递给 ATP 敏感性钾通道。  |  Carrasco, AJ., et al. 2001. Proc Natl Acad Sci U S A. 98: 7623-8. PMID: 11390963
  3. 腺苷酸激酶 2,一种线粒体酶。  |  Bruns, GA. and Regina, VM. 1977. Biochem Genet. 15: 477-86. PMID: 195572
  4. 人类腺苷酸激酶 2A cDNA 的克隆和特征描述。  |  Lee, Y., et al. 1996. Biochem Mol Biol Int. 39: 833-42. PMID: 8843353
  5. 编码人类腺苷酸激酶同工酶 2 基因的 cDNA 克隆和组织特异性表达。  |  Noma, T., et al. 1998. Biochim Biophys Acta. 1395: 34-9. PMID: 9434148
  6. 人类腺苷酸激酶 2 同工酶的克隆和表达:腺苷酸激酶 1 和 2 在人类肌肉组织中的差异表达。  |  Lee, Y., et al. 1998. J Biochem. 123: 47-54. PMID: 9504408
  7. 腺苷酸激酶:在完整细胞中的动力学行为表明它与多种细胞过程密不可分。  |  Dzeja, PP., et al. 1998. Mol Cell Biochem. 184: 169-82. PMID: 9746320

订购信息

产品名称产品编号规格价格数量收藏夹

AK2 抗体 (F-2)

sc-374095
200 µg/ml
$316.00

AK2 (F-2): m-IgG Fc BP-HRP 套装

sc-529658
200 µg Ab; 10 µg BP
$354.00

AK2 (F-2): m-IgGκ BP-HRP 套装

sc-522619
200 µg Ab, 40 µg BP
$354.00

AK2 (F-2): m-IgG1 BP-HRP 套装

sc-543626
200 µg Ab; 20 µg BP
$354.00

AK2 抗体 (F-2) AC

sc-374095 AC
500 µg/ml, 25% agarose
$416.00

AK2 抗体 (F-2) HRP

sc-374095 HRP
200 µg/ml
$316.00

AK2 抗体 (F-2) FITC

sc-374095 FITC
200 µg/ml
$330.00

AK2 抗体 (F-2) PE

sc-374095 PE
200 µg/ml
$343.00

AK2 抗体 (F-2) Alexa Fluor® 488

sc-374095 AF488
200 µg/ml
$357.00

AK2 抗体 (F-2) Alexa Fluor® 546

sc-374095 AF546
200 µg/ml
$357.00

AK2 抗体 (F-2) Alexa Fluor® 594

sc-374095 AF594
200 µg/ml
$357.00

AK2 抗体 (F-2) Alexa Fluor® 647

sc-374095 AF647
200 µg/ml
$357.00

AK2 抗体 (F-2) Alexa Fluor® 680

sc-374095 AF680
200 µg/ml
$357.00

AK2 抗体 (F-2) Alexa Fluor® 790

sc-374095 AF790
200 µg/ml
$357.00

I am using AK2 (F-2): sc-374095 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Nice band at expected molecular weightTested in WB on mouse purified mitochondria and there was a nice band at the expected MW + an aspecific band at about 97 KDa
Date published: 2018-01-17
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Highly potent and specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~25 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-10
Rated 5 out of 5 by from Satisfactory Western Blot data of AK2 expressionSatisfactory Western Blot data of AK2 expression in HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-12-01
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AK2 抗体 (F-2) is rated 5.0 out of 5 by 5.
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