Date published: 2025-9-5
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AGPS Antibody (A-2) - Western Blotting - Image 403572
AGPS 抗体 (A-2): sc-374201. RT-4 (A), SW480 (B), KNRK (C) 和 Hep G2 (D) 全细胞裂解液中 AGPS 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.
AGPS 抗体 (A-2): sc-374201. RT-4 (A), SW480 (B), KNRK (C) 和 Hep G2 (D) 全细胞裂解液中 AGPS 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 790: sc-516181.

AGPS 抗体 (A-2): sc-374201

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说明书
  • AGPS 抗体 A-2 是小鼠单克隆 IgG1 κ，AGPS抗体, 在9篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的AGPS的C-terminus附近的氨基酸577-609之间
  • AGPS 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AGPS; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗AGPS抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AGPS (A-2): sc-374201的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是AGPS Antibody (A-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与AGPS Antibody (A-2) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

AGPS抗体（A-2）是一种IgG1 κ小鼠单克隆AGPS抗体（也称为AGPS抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的AGPS蛋白。AGPS抗体（A-2）既有非偶联抗AGPS抗体形式，也有多种偶联形式的抗AGPS抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。AGPS（烷基二羟基丙酮磷酸合酶），也被称为烷基甘油磷酸合酶和AAG5（衰老相关基因5蛋白），是一种含有658个氨基酸的酶，对甘油酯代谢和醚脂生物合成至关重要。AGPS定位于过氧化物酶体膜的内侧，可能是由GNPAT和一种GNPAT修饰形式组成的异源三聚体复合物的一部分。AGPS含有一个FAD结合PCMH型结构域，在合成烷基-甘油-3-磷酸和长链酸阴离子时，以FAD为辅因子，原料为1-乙酰基-甘油-3-磷酸和长链醇。编码AGPS的基因缺陷会导致3型点状根性软骨发育不良，这是一种以椎体疾病、严重智力迟钝、皮肤病变、白内障以及肱骨和股骨的根性缩短为特征的疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AGPS 抗体 (A-2) 参考文献:

  1. 烷基二羟基丙酮磷酸合成酶和二羟基丙酮磷酸酰基转移酶在过氧物酶体中形成一个蛋白质复合物。  |  Biermann, J., et al. 1999. Eur J Biochem. 261: 492-9. PMID: 10215861
  2. 醚脂生物合成缺陷中的膜运输障碍  |  Thai, TP., et al. 2001. Hum Mol Genet. 10: 127-36. PMID: 11152660
  3. 烷基二羟基丙酮磷酸合成酶。  |  de Vet, EC. and van den Bosch, H. 2000. Cell Biochem Biophys. 32 Spring: 117-21. PMID: 11330037
  4. 烷基二羟基乙酰丙酮磷酸合成酶缺陷突变动物细胞系的分离与鉴定：质氨酸在高尔基体后区室中的定位和转运  |  Honsho, M., et al. 2008. Biochim Biophys Acta. 1783: 1857-65. PMID: 18571506
  5. 赖氨酸乙酰化以蛋白质复合物为目标，并共同调节主要的细胞功能。  |  Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
  6. 内质网中含烷基 GPI-anchor 生物合成的过氧化物酶体依赖性。  |  Kanzawa, N., et al. 2009. Proc Natl Acad Sci U S A. 106: 17711-6. PMID: 19815513
  7. 人类烷基二羟基丙酮磷酸合成酶 cDNA 的核苷酸序列显示存在过氧物酶体靶向信号 2。  |  de Vet, EC., et al. 1997. Biochim Biophys Acta. 1346: 25-9. PMID: 9187299
  8. 烷基二羟基丙酮磷酸合成酶。过氧化物酶体生物发生障碍的命运以及单酶缺乏症的点突变的鉴定。  |  de Vet, EC., et al. 1998. J Biol Chem. 273: 10296-301. PMID: 9553082

订购信息

产品名称产品编号规格价格数量收藏夹

AGPS 抗体 (A-2)

sc-374201
200 µg/ml
$316.00

AGPS (A-2): m-IgG Fc BP-HRP 套装

sc-552023
200 µg Ab; 10 µg BP
$354.00

AGPS 抗体 (A-2) AC

sc-374201 AC
500 µg/ml, 25% agarose
$416.00

AGPS 抗体 (A-2) HRP

sc-374201 HRP
200 µg/ml
$316.00

AGPS 抗体 (A-2) FITC

sc-374201 FITC
200 µg/ml
$330.00

AGPS 抗体 (A-2) PE

sc-374201 PE
200 µg/ml
$343.00

AGPS 抗体 (A-2) Alexa Fluor® 488

sc-374201 AF488
200 µg/ml
$357.00

AGPS 抗体 (A-2) Alexa Fluor® 546

sc-374201 AF546
200 µg/ml
$357.00

AGPS 抗体 (A-2) Alexa Fluor® 594

sc-374201 AF594
200 µg/ml
$357.00

AGPS 抗体 (A-2) Alexa Fluor® 647

sc-374201 AF647
200 µg/ml
$357.00

AGPS 抗体 (A-2) Alexa Fluor® 680

sc-374201 AF680
200 µg/ml
$357.00

AGPS 抗体 (A-2) Alexa Fluor® 790

sc-374201 AF790
200 µg/ml
$357.00

AGPS (A-2) 中和勝肽

sc-374201 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-374201 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Ab can be used ot investigage peroxisomal functionWe used the sc-374201 Alkylglyceronephosphate synthase (AGPS) antibody to investigate peroxisomal functions using western blotting. The AGPS ab used showed a clear and specific band in western blot that could be excellentiy used for quantification. AGPS protein expression was significantly higher compared to the expression of another testet protein.
Date published: 2022-03-24
Rated 5 out of 5 by from works well for WBresults showed very good specificity in WB of human lung cancer cells
Date published: 2017-05-27
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-01
Rated 5 out of 5 by from this antibody works very excellent for WBthis antibody works very excellent for WB. This antibody doesn t stain for lung tissues of mouse and human origin (IF didn t work). IF is not good though as mentioned from the data sheet as cytoplasmic staining, but this should stain peroxisomes, often they are clubbed and had lot of background staining.
Date published: 2016-01-18
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-14
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AGPS 抗体 (A-2) is rated 4.8 out of 5 by 6.
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