Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ADK Double Nickase Plasmid (h): sc-403273-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ADK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ADK Double Nickase Plasmid (h) and ADK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ADK Antibody (H-1): sc-514588
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADK Double Nickase Plasmid (h) | sc-403273-NIC | 20 µg | $410.00 | |||
ADK Double Nickase Plasmid (h2) | sc-403273-NIC-2 | 20 µg | $410.00 |
Adenosine kinase (ADK) is a key regulator of purine salvage that phosphorylates adenosine to AMP, thereby controlling intracellular adenosine availability and influencing the adenylate pool. By modulating adenosine metabolism, ADK impacts energy homeostasis and signaling processes linked to nucleotide turnover and stress responses. Altered ADK activity can shift adenosine-dependent pathways that affect inflammation, neuromodulatory signaling, and cellular excitability, making it relevant to studies of neurological and metabolic phenotypes. In human systems, ADK is frequently used as a node to interrogate how purine metabolism interfaces with cell state changes under hypoxia, oxidative stress, or altered mitochondrial function.
ADK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.