Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h): sc-402004-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h) and Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Adenylate cyclase 8/AC8/ADCY8 Antibody (B-6): sc-377323
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h) | sc-402004-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h2) | sc-402004-NIC-2 | 20 µg | $410.00 |
Human ADCY8 encodes adenylate cyclase 8 (AC8), a membrane-bound, Ca2+/calmodulin-stimulated enzyme that converts ATP to cAMP and couples calcium signals to second-messenger production. AC8-driven cAMP activates PKA, EPAC, and cyclic nucleotide–gated pathways, shaping CREB-dependent transcription, synaptic plasticity, and stimulus-secretion coupling in excitable tissues. Through integration of GPCR inputs and intracellular Ca2+ dynamics, ADCY8 contributes to regulation of neuronal signaling, endocrine responses, and adaptive cellular homeostasis. Altered ADCY8 expression or signaling has been implicated in neuropsychiatric and cognitive phenotypes and is studied in the context of dysregulated cAMP signaling observed across neurological and metabolic disease research.
Adenylate cyclase 8/AC8/ADCY8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.