Date published: 2025-9-5
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ADAM10 Antibody (B-3) - Western Blotting - Image 9539
ADAM10 抗体 (B-3): sc-28358. U-937 (A), HuT 78 (B), NIH/3T3 (C), MH-S (D) 和 KNRK (E) 全细胞裂解液中 ADAM10 表达的 Western 印迹分析.
ADAM10 抗体 (B-3): sc-28358. U-937 (A), HuT 78 (B), NIH/3T3 (C), MH-S (D) 和 KNRK (E) 全细胞裂解液中 ADAM10 表达的 Western 印迹分析.

ADAM10 抗体 (B-3): sc-28358

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寫評論提問

说明书
  • ADAM10 抗体 B-3 是小鼠单克隆 IgG2a κ，ADAM10抗体, 在27篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ADAM10的氨基酸1-300
  • ADAM10 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ADAM10
  • 抗ADAM10抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ADAM10 (B-3): sc-28358的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是ADAM10 Antibody (B-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ADAM10 Antibody (B-3) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外ADAM抗体，包括ADAMD, ADAM10, ADAM12, ADAM15, ADAM19, ADAM22, ADAM26, ADAM28, ADAM3, ADAM32, ADAM33, ADAM4, ADAM5, ADAM8, ADAM9和ADAMDEC1

ADAM10抗体（B-3）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的ADAM10蛋白。抗ADAM10抗体（B-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。ADAM10是ADAM（一种组织蛋白酶和金属蛋白酶）家族的一员，在处理肿瘤坏死因子α（TNFα）方面发挥着关键作用，而TNFα是一种参与炎症和免疫反应的关键细胞因子。通过在Ala 76-Val 77位点切割膜结合的前体促肿瘤坏死因子，ADAM10促进可溶性TNF的释放，而可溶性TNF对于调节炎症过程和介导细胞信号通路至关重要。ADAM10的正常运作对于维持包括神经发生和细胞通讯在内的各种生理过程中的稳态至关重要，这使得ADAM10成为癌症、神经退行性疾病和免疫失调研究的重要目标。ADAM10单克隆抗体（B-3）是研究人员探索ADAM10在健康和疾病中复杂作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ADAM10 抗体 (B-3) 参考文献:

  1. 含分解蛋白样和金属蛋白酶的 ADAM 家族的结构-功能分析（综述）。  |  Stone, AL., et al. 1999. J Protein Chem. 18: 447-65. PMID: 10449042
  2. ADAM 基因家族：具有粘附和蛋白酶活性的表面蛋白。  |  Primakoff, P. and Myles, DG. 2000. Trends Genet. 16: 83-7. PMID: 10652535
  3. ADAM10在阿尔茨海默病中的作用。  |  Yuan, XZ., et al. 2017. J Alzheimers Dis. 58: 303-322. PMID: 28409746
  4. 癌症和自身免疫的 ADAM10 靶向治疗。  |  Smith, TM., et al. 2020. Front Immunol. 11: 499. PMID: 32265938
  5. ADAM10 切割 Trop-2 是癌症生长和转移的激活开关。  |  Trerotola, M., et al. 2021. Neoplasia. 23: 415-428. PMID: 33839455
  6. TspanC8家族中的跨膜蛋白调节ADAM10及其治疗潜力。  |  Harrison, N., et al. 2021. Int J Mol Sci. 22: PMID: 34201472
  7. ADAM10裂解的ephrin-A5有助于前列腺癌转移。  |  Cai, C., et al. 2022. Cell Death Dis. 13: 453. PMID: 35551177
  8. 肾病的 ADAM10 靶向治疗。  |  Wang, JN. and Cao, XJ. 2023. Curr Mol Med. 23: 1037-1045. PMID: 36278450
  9. 内皮细胞ADAM10的利用确定了细菌性败血症小鼠血管损伤的分子途径。  |  Alfano, DN., et al. 2023. J Clin Invest. 133: PMID: 37788087
  10. 凋亡介导的ADAM10激活消除了粘蛋白屏障，促进了T细胞外泌体。  |  Drexhage, LZ., et al. 2024. Nat Commun. 15: 541. PMID: 38225245
  11. ADAM，一种含有崩解蛋白和金属蛋白酶结构域的新型膜蛋白家族：在细胞-细胞和细胞-基质相互作用中具有多种潜在功能。  |  Wolfsberg, TG., et al. 1995. J Cell Biol. 131: 275-8. PMID: 7593158
  12. ADAM锌金属蛋白酶家族中一种促肿瘤坏死因子-α加工酶的鉴定和表征。  |  Rosendahl, MS., et al. 1997. J Biol Chem. 272: 24588-93. PMID: 9305925

订购信息

产品名称产品编号规格价格数量收藏夹

ADAM10 抗体 (B-3)

sc-28358
200 µg/ml
$316.00

ADAM10 (B-3): m-IgG Fc BP-HRP 套装

sc-525525
200 µg Ab; 10 µg BP
$354.00

ADAM10 (B-3): m-IgG2a BP-HRP 套装

sc-546304
200 µg Ab; 10 µg BP
$354.00

ADAM10 抗体 (B-3) AC

sc-28358 AC
500 µg/ml, 25% agarose
$416.00

ADAM10 抗体 (B-3) HRP

sc-28358 HRP
200 µg/ml
$316.00

ADAM10 抗体 (B-3) FITC

sc-28358 FITC
200 µg/ml
$330.00

ADAM10 抗体 (B-3) PE

sc-28358 PE
200 µg/ml
$343.00

ADAM10 抗体 (B-3) Alexa Fluor® 488

sc-28358 AF488
200 µg/ml
$357.00

ADAM10 抗体 (B-3) Alexa Fluor® 546

sc-28358 AF546
200 µg/ml
$357.00

ADAM10 抗体 (B-3) Alexa Fluor® 594

sc-28358 AF594
200 µg/ml
$357.00

ADAM10 抗体 (B-3) Alexa Fluor® 647

sc-28358 AF647
200 µg/ml
$357.00

ADAM10 抗体 (B-3) Alexa Fluor® 680

sc-28358 AF680
200 µg/ml
$357.00

ADAM10 抗体 (B-3) Alexa Fluor® 790

sc-28358 AF790
200 µg/ml
$357.00

Can you recommend a positive control to use with this antibody, sc-28358, for Western blot?

Asked by: TinTin
We recommend HeLa: sc-2200, NIH/3T3: sc-2210 or AMJ2-C8: sc-364366 whole cell lysates to use as positive controls as we have Western blot data showing specific detection of FGFR-4 in these cell lines.
Answered by: Technical Support 4
Date published: 2017-01-16
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Rated 5 out of 5 by from Only a band in breast cancer cellsThe an,body was diluted at 1:1000 in 3%BSA and used ON. The filter was then incubated with a secondary an,body (used 1:3000 in 5%Milk for 1h). It is visible only a band migra,ng at about 100 KDa. The western blot is very clear. These are MDA-MB 231 cells.
Date published: 2020-07-21
Rated 1 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and did not show a signal, even at dilution 1:25
Date published: 2018-11-16
Rated 5 out of 5 by from works properlyI got this as a free sample to try. The band showed up where it should be and in general, it works properly as it indicated. It does need a little extra washing/blocking as I noticed.
Date published: 2017-10-30
Rated 5 out of 5 by from greatI used this antibody for WB to detect endogenous ADAM10 in mice brain tissue samples, it has specific bands on 75kd
Date published: 2017-09-05
Rated 4 out of 5 by from work well for WBThe bands near the target protein are very good and the specificity is very strong by WB experiments.
Date published: 2017-07-17
Rated 5 out of 5 by from Good antibody for WBADAM10 (B-3) is a good antibody fo WB, and it detects very strong band.
Date published: 2017-03-30
Rated 5 out of 5 by from it detects very strong band ~75kDaAlthough the antibody detect several non-specific bands, it detects very strong band ~75kDa. Probably need very low antibody amount for mouse bran lysate.
Date published: 2017-02-16
Rated 5 out of 5 by from Strong westernVery nice wester blot data; strong signal with low back ground
Date published: 2017-01-26
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ADAM10 抗体 (B-3) is rated 4.6 out of 5 by 19.
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