Date published: 2025-9-6
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Actin Antibody (C-2) - Western Blotting - Image 350859
肌动蛋白抗体 (C-2): sc-8432. C32 (A), A-431 (B), Sol8 (C), BC3H1 (D) 和 RAW 264. 7 (E) 全细胞裂解液中肌动蛋白表达的 Western 印迹分析.
肌动蛋白抗体 (C-2): sc-8432. C32 (A), A-431 (B), Sol8 (C), BC3H1 (D) 和 RAW 264. 7 (E) 全细胞裂解液中肌动蛋白表达的 Western 印迹分析.

Actin 抗体 (C-2): sc-8432

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说明书
  • Actin 抗体 C-2 是小鼠单克隆 IgG1 κ，Actin抗体, 在2,630篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Actin的C-terminus的氨基酸357-375之间
  • Actin 抗体 (C-2) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA， 检测 mouse, rat, human, 和 Xenopus 来源的 a broad range of Actin isoforms; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Actin抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Actin (C-2): sc-8432的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Actin 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Actin 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Actin抗体，包括α-Actin, β-Actin, γ-Actin, Muscle Actin和Smooth Muscle Actin

肌动蛋白抗体（C-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可特异性检测小鼠、大鼠、人类和非洲爪蟾物种中的肌动蛋白，这是一种高度保守且必不可少的细胞骨架蛋白。抗肌动蛋白抗体（C-2）的表位位于人源肌动蛋白C端357-375氨基酸之间，可用于多种检测方法，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。肌动蛋白单克隆抗体（C-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®染料。肌动蛋白是一种普遍存在于真核细胞中的蛋白质，占细胞总蛋白的50%。肌动蛋白在形成稳定和动态结构（如微绒毛和肌肉细胞的收缩装置）中发挥着重要作用，这凸显了肌动蛋白在细胞结构和运动中的重要性。在哺乳动物中，至少存在六种肌动蛋白同种型，分为三大类：α-肌动蛋白主要存在于肌肉细胞中，而β-肌动蛋白和γ-肌动蛋白存在于大多数其他组织中。肌动蛋白在细胞分裂、细胞内运输和信号转导等细胞过程中发挥着关键作用。细胞骨架中的肌动蛋白组织受到小GTP酶家族成员的严格调控，包括控制应力纤维组装的Rho、调节质膜相关丝的Rac以及促进丝状突起形成的Cdc42。肌动蛋白在维持细胞结构和促进细胞动态反应方面发挥着关键作用，因此成为研究基本生物过程和疾病的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Actin 抗体 (C-2) 参考文献:

  1. 基于肌动蛋白的运动：从分子到运动。  |  Carlier, MF., et al. 2003. Bioessays. 25: 336-45. PMID: 12655641
  2. Vav1 通过调节肌动蛋白和微管动力学控制 DAP10 介导的天然细胞毒性。  |  Graham, DB., et al. 2006. J Immunol. 177: 2349-55. PMID: 16887996
  3. [平滑肌α-肌动蛋白在慢性肾小球肾炎患者肾纤维化发展过程中的作用]。  |  Chebotareva, NV., et al. 2006. Ter Arkh. 78: 17-21. PMID: 16889044
  4. 评估肌动蛋白的生长速率。  |  Short, B. 2015. J Cell Biol. 208: 499. PMID: 25733710
  5. 微管相关蛋白在控制微管组装中的作用  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
  6. Rho, rac和cdc42 GTP酶调节与肌动蛋白应力纤维, 薄片和丝状体相关的多分子缔合作用。  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
  7. 关于 F-肌动蛋白建模的讨论。  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
  8. 由微丝蛋白稳定化的动态肌动蛋白结构。  |  Finkel, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 1510-4. PMID: 8108438
  9. 肌动蛋白细胞骨架设定细胞运动的节奏  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
  10. 真核生物蛋白质的起源和进化。  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
  11. 弹性纤维是人类胎盘干绒毛基质的重要组成部分，也是血管周围收缩鞘的组成部分。  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
  12. 激光共聚焦显微镜揭示的人类毛囊中的肌动蛋白束。  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

订购信息

产品名称产品编号规格价格数量收藏夹

Actin 抗体 (C-2)

sc-8432
200 µg/ml
$316.00

Actin (C-2): m-IgG Fc BP-HRP 套装

sc-526525
200 µg Ab; 10 µg BP
$354.00

Actin (C-2): m-IgG1 BP-HRP 套装

sc-531898
200 µg Ab; 20 µg BP
$354.00

Actin 抗体 (C-2) AC

sc-8432 AC
500 µg/ml, 25% agarose
$416.00

Actin 抗体 (C-2) HRP

sc-8432 HRP
200 µg/ml
$316.00

Actin 抗体 (C-2) FITC

sc-8432 FITC
200 µg/ml
$330.00

Actin 抗体 (C-2) PE

sc-8432 PE
200 µg/ml
$343.00

Actin 抗体 (C-2) Alexa Fluor® 488

sc-8432 AF488
200 µg/ml
$357.00

Actin 抗体 (C-2) Alexa Fluor® 546

sc-8432 AF546
200 µg/ml
$357.00

Actin 抗体 (C-2) Alexa Fluor® 594

sc-8432 AF594
200 µg/ml
$357.00

Actin 抗体 (C-2) Alexa Fluor® 647

sc-8432 AF647
200 µg/ml
$357.00

Actin 抗体 (C-2) Alexa Fluor® 680

sc-8432 AF680
200 µg/ml
$357.00

Actin 抗体 (C-2) Alexa Fluor® 790

sc-8432 AF790
200 µg/ml
$357.00

Actin 抗体 (C-2) TRITC

sc-8432 TRITC
200 µg/ml
$320.00

Actin 抗体 (C-2) Alexa Fluor® 405

sc-8432 AF405
200 µg/ml
$357.00

Actin (C-2) 中和勝肽

sc-8432 P
100 µg/0.5 ml
$68.00

As you may know that the publications in higher rank of Journals request the list of antibody with RRID number. For example, your product with Cat# sc-101544. The correspondent RRID is AB2157327. Where I can find RRID while I purchase your antibodies??

Asked by: Ka Bian
Thank you for your question. SCBT is not responsible for the curation or assignment of RRID numbers for our products. This is an initiative from a consortium of researchers and The Antibody Registry.
Answered by: BlakeJ
Date published: 2023-09-25

Hello, we are currently using the actin sc-8432 HPR antibody for WB and are seeing three bands instead of one. Do you have any troubleshooting advice on how to resolve this issue?

Asked by: Brittnee
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-11-09

Hello, I’d like to test if this antibody works on several species of foraminifera (Quinqueloculina sp., Ammonia sp., Amphistegina lessonii). How can I apply for the trial sample?

Asked by: Jan P Golen
Thank you for your question. Please contact our Technical Service Department (or your local Distributor) directly to arrange for a free 10 µg sample of Actin (C-2): sc-8432.
Answered by: Tech Support Europe
Date published: 2022-01-19

An antibody for F-actin detection?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-03-16

Potrei sapere qual è il volume in cui viene fornito l'anticorpo anti-actina C2 Sc8432? Grazie mille

Asked by: Federica10
Thank you for your question. This actin antiobdy, sc-8432, is provided as 200µg in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Tech Service
Date published: 2019-09-10

What is the recommended dilution buffer for this antibody?

Asked by: billnye
Thank you for your question. We recommend UltraCruz® Blocking Reagent: sc-516214 ready-to-use solution for blocking and dilution, our animal-free alternative to sera, BSA or non-fat dry milk.
Answered by: Tech Support Europe
Date published: 2023-01-04

what is the dilution for the Western blot

Asked by: niezu
We recommend a starting dilution of 1:200, with a range of 1:100-1:1,000.
Answered by: Technical Support
Date published: 2023-01-04

Would this antibody recognize actin in filament (F-actin) or subunit (G-actin)?

Asked by: Anonymous
Thank you for your question. This antibody recognizes a broad range of Actin isoforms, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin
Answered by: Technical Support
Date published: 2021-09-08
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Rated 5 out of 5 by from 量很多，而且跑的条带很清晰，分辨率高课题组一直用的Santa Cruze的内参actin,在细胞和动物的样品上都有很好的条带。杂带也比较少!
Date published: 2025-07-28
Rated 5 out of 5 by from works well and as describedworks well and as described, began with concentration recommended on data sheet and it worked
Date published: 2025-03-10
Rated 5 out of 5 by from Good antibody for western blotgood antibody for western blot without any cross-link bands.
Date published: 2023-10-12
Rated 1 out of 5 by from No hubo reconocimiento de la proteinaLo utilicé para el reconocimiento de la proteína en WB en una línea celular de mosquito (C6/36) y no hubo presencia de la banda esperada
Date published: 2023-10-09
Rated 5 out of 5 by from 产品好用价格实惠 条带很清晰，抗体结合效果好，为科研省时省力，建议购买使用，种属即使是禽类 结合的也很不错 。
Date published: 2023-03-03
Rated 1 out of 5 by from Actin Ab for western blotting.Did not work on CRC cell lines. Dilutions used was 1 in 250 in 5% BSA/TBST. Loading 30 ug of whole protein lysate.
Date published: 2023-01-24
Rated 4 out of 5 by from The beta actinThe beta actin we used in our work also works very well diluted 1:2000. it was used on placental tissue samples and astrocyte cell cultures. I highly recommend it
Date published: 2023-01-11
Rated 5 out of 5 by from 很好特异性很强商品试用很好用、试用装给我们使用体验，用完好用就会放心购买。用了试用装很不错，会继续购买正装用于科研。
Date published: 2021-12-06
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Actin 抗体 (C-2) is rated 4.5 out of 5 by 85.
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