ACAD-9 CRISPR/Cas9 KO Plasmid (m): sc-432841
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ACAD-9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ACAD-9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACAD-9 CRISPR/Cas9 KO Plasmid (m) | sc-432841 | 20 µg | $397.00 |
Overview
Acad9 encodes acyl-CoA dehydrogenase family member 9 (ACAD-9), a mitochondrial flavoprotein that supports fatty acid β-oxidation and is required for efficient assembly of oxidative phosphorylation complex I. Through its roles in mitochondrial electron transfer and energy metabolism, ACAD-9 influences redox homeostasis, ATP production, and metabolic flexibility in high-demand tissues. Disruption of ACAD9 function is associated with complex I assembly defects and mitochondrial encephalomyopathy-like phenotypes, making it relevant for studying bioenergetic failure, metabolic stress responses, and mitochondrial disease mechanisms in mouse models.
ACAD-9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acad9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acad9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acad9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ACAD-9 protein expression.
This CRISPR knockout system enables efficient generation of Acad9-deficient cell models for investigation of ACAD-9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acad9 exon(s) critical for ACAD-9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acad9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ACAD-9 CRISPR/Cas9 KO Plasmid (m) and ACAD-9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acad9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ACAD-9 HDR Plasmid (m) and ACAD-9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acad9 homology arms to support homology-directed repair at defined Acad9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.