IFN-γRβ Antibody (MOB-47): sc-12752

IFN-γRβ Antibody (MOB-47) is a hamster monoclonal IgG antibody that detects IFN-γRβ in mouse samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). IFN-γRβ, also known as the signal transducing component of the interferon-gamma receptor, plays a crucial role in mediating the biological effects of interferon-gamma, including antiviral, antiproliferative, and immunomodulatory responses. IFN-γRβ is located on the cell surface, where IFN-γRβ forms a functional receptor complex with IFN-γRα, enabling the binding of interferon-gamma and subsequent activation of intracellular signaling pathways. The importance of IFN-γRβ′s location lies in IFN-γRβ′s ability to initiate a cascade of events that lead to the transcriptional activation of genes involved in immune responses, thereby influencing the overall immune system′s ability to respond to pathogens and tumors. The interaction of IFN-γRβ with Janus kinases JAK1 and JAK2 upon receptor activation is essential for the phosphorylation and nuclear translocation of transcription factors such as Stat1, which are critical for the expression of interferon-gamma-inducible genes. The proper functioning of this receptor complex is vital for effective immune surveillance and response, making IFN-γRβ monoclonal antibody (MOB-47) an invaluable tool for researchers studying immune signaling pathways and their implications in disease.

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IFN-γRβ Antibody (MOB-47) References:

1. p300 mediates functional synergism between AF-1 and AF-2 of estrogen receptor alpha and beta by interacting directly with the N-terminal A/B domains.  |  Kobayashi, Y., et al. 2000. J Biol Chem. 275: 15645-51. PMID: 10747867
2. Differential nuclear localization of the IFNGR-1 and IFNGR-2 subunits of the IFN-gamma receptor complex following activation by IFN-gamma.  |  Larkin, J., et al. 2000. J Interferon Cytokine Res. 20: 565-76. PMID: 10888113
3. The AF-1 and AF-2 activating domains of retinoic acid receptor-alpha (RARalpha) and their phosphorylation are differentially involved in parietal endodermal differentiation of F9 cells and retinoid-induced expression of target genes.  |  Rochette-Egly, C., et al. 2000. Mol Endocrinol. 14: 1398-410. PMID: 10976918
4. Differential contributions of AF-1 and AF-2 activities to the developmental functions of RXR alpha.  |  Mascrez, B., et al. 2001. Development. 128: 2049-62. PMID: 11493527
5. Vinexin beta interacts with the non-phosphorylated AF-1 domain of retinoid receptor gamma (RARgamma) and represses RARgamma-mediated transcription.  |  Bour, G., et al. 2005. J Biol Chem. 280: 17027-37. PMID: 15734736
6. Lessons from the dissection of the activation functions (AF-1 and AF-2) of the estrogen receptor alpha in vivo.  |  Arnal, JF., et al. 2013. Steroids. 78: 576-82. PMID: 23200732
7. N-terminal transactivation function, AF-1, of estrogen receptor alpha controls obesity through enhancement of energy expenditure.  |  Arao, Y., et al. 2018. Mol Metab. 18: 68-78. PMID: 30287090
8. Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium.  |  Cagnet, S., et al. 2018. Nat Commun. 9: 4723. PMID: 30413705
9. Melatonergic signalling instructs transcriptional inhibition of IFNGR2 to lessen interleukin-1β-dependent inflammation.  |  Xia, Y., et al. 2022. Clin Transl Med. 12: e716. PMID: 35184395
10. Genomic organization and promoter analysis of the gene ifngr2 encoding the second chain of the mouse interferon-gamma receptor.  |  Ebensperger, C., et al. 1996. Scand J Immunol. 44: 599-606. PMID: 8972742

The preferred IFN-γRβ antibody is IFN-γRβ Antibody (A-11): sc-377291.