cytochrome c Antibody (A-8): sc-13156

cytochrome c Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody raised against amino acids 1-104 of equine cytochrome c, reactive with mouse, rat, and human proteins. cytochrome c, also known as CYCS or somatic cytochrome c, is a small heme protein located in the mitochondrial intermembrane space. Such localization allows cytochrome c to play a key role in the electron transport chain, transferring electrons between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase), which is essential for ATP production during oxidative phosphorylation. cytochrome c′s position in the mitochondria enables participation in the intrinsic pathway of apoptosis; upon release into the cytosol, cytochrome c interacts with apoptotic protease activating factor-1 (Apaf-1) to form the apoptosome, leading to the activation of caspase-9 and downstream effector caspases like caspase-3. Release of cytochrome c from mitochondria is a pivotal event in programmed cell death, and dysregulation of this process is associated with diseases such as cancer and neurodegenerative disorders. cytochrome c monoclonal Antibody (A-8) is suitable for applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry on paraffin-embedded sections, flow cytometry (FCM), and ELISA. Researchers utilize cytochrome anti-c Antibody (A-8) to study mitochondrial function, apoptosis mechanisms, and related cellular processes, providing insights into energy metabolism and cell death pathways essential for understanding various physiological and pathological conditions.

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cytochrome c Antibody (A-8) References:

1. Protonmotive cooperativity in cytochrome c oxidase.  |  Papa, S., et al. 2004. Biochim Biophys Acta. 1658: 95-105. PMID: 15282180
2. Mechanisms of cytochrome c release from mitochondria.  |  Garrido, C., et al. 2006. Cell Death Differ. 13: 1423-33. PMID: 16676004
3. In vitro susceptibility of Staphylococcus aureus to thrombin-induced platelet microbicidal protein-1 (tPMP-1) is influenced by cell membrane phospholipid composition and asymmetry.  |  Mukhopadhyay, K., et al. 2007. Microbiology (Reading). 153: 1187-1197. PMID: 17379728
4. Biogenesis of mitochondrial c-type cytochromes.  |  Gonzales, DH. and Neupert, W. 1990. J Bioenerg Biomembr. 22: 753-68. PMID: 1965436
5. Cytochrome c Stabilization and Immobilization in Aerogels.  |  Harper-Leatherman, AS., et al. 2017. Methods Mol Biol. 1504: 149-163. PMID: 27770420
6. Interaction of Cytochrome C Oxidase with Steroid Hormones.  |  Oleynikov, IP., et al. 2020. Cells. 9: PMID: 33003582
7. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c.  |  Liu, X., et al. 1996. Cell. 86: 147-57. PMID: 8689682
8. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
9. The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis.  |  Kluck, RM., et al. 1997. Science. 275: 1132-6. PMID: 9027315
10. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.  |  Zou, H., et al. 1997. Cell. 90: 405-13. PMID: 9267021
11. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade.  |  Li, P., et al. 1997. Cell. 91: 479-89. PMID: 9390557
12. Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c.  |  Rossé, T., et al. 1998. Nature. 391: 496-9. PMID: 9461218

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cytochrome c Antibody (A-8) has 565 citations in a variety of scientific publications.

Primary antibodies like Anti-cytochrome c Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cytochrome c Antibody (A-8) Specifications

Alternate Product Name: cytochrome c

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-104

Epitope Target: cytochrome c

Epitope Species: equine

Product Reactivity/Detection: cytochrome c

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 565 citations in various scientific publications

Available Conjugates/Forms for cytochrome c Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin