7-Amino-4-methylcoumarin (CAS 26093-31-2)

7-Amino-4-methylcoumarin, with the CAS number 26093-31-2, is a chemical compound known for its fluorescent properties, which makes it particularly valuable in the field of biochemical research. Structurally, it belongs to the coumarin family, which is characterized by a benzopyrone ring system that exhibits strong fluorescence. This compound is particularly notable for its ability to emit blue fluorescence when excited by ultraviolet light, making it an excellent fluorophore for various applications in molecular biology and chemistry. The specific mechanism of action of 7-amino-4-methylcoumarin involves its ability to absorb light at a certain wavelength and subsequently emit light at a longer wavelength, a process known as fluorescence. This property is exploited in research methodologies such as fluorescence microscopy, where it can be conjugated to antibodies or other probes to visualize the presence and distribution of target molecules within cells or tissues. Additionally, its fluorescence intensity and stability under physiological conditions allow for detailed imaging and quantitative analysis. Researchers also leverage 7-amino-4-methylcoumarin in the development of fluorescent sensors and markers, where its ability to respond to changes in the environment by altering its fluorescent properties is particularly valuable for real-time monitoring of various biological processes.