YAP Antibody (H-9) recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

YAP Antibody (H-9): sc-271134

YAP Antibody (H-9) is rated 4.7 out of 5 by 18.
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Datasheets
    • YAP Antibody (H-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 379-407 near the C-terminus of YAP of human origin
    • recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-271134 X, 200 µg/0.1 ml)
    • blocking peptide, sc-271134 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of YAP (H-9): sc-271134.
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What secondary reagent should I use for IHC if I am working with mice tissue samples?

Asked by: Trav11
For cases like this, we recommend using our mouse monoclonal primary antibodies that are directly conjugated. The YAP Antibody (H-9): sc-271134 is NOT currently available directly conjugated, but we do offer an alternative, YAP Antibody (G-6): sc-376830, that is directly conjugated. Currently, sc-376830 is available conjugated to HRP (sc-376830 HRP), phycoerythrin (sc-376830 PE), fluorescein (sc-376830 FITC), Alexa Fluor® 488 (sc-376830 AF488) or Alexa Fluor® 647 (sc-376830 AF647).
Answered by: Tech Service 8
Date published: 2016-12-22

Dear company, Could you please help me to consult the price and inventory of the product YAP Antibody (H-9): sc-271134.Many thanks. Yours, Grace

Asked by: Grace Li
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Rated 3 out of 5 by from Bright cytosolic staining, limited nuclear stainin MDCKs fixed in 4% paraformaldehyde, stained with primary at 1:100 3 hr at 37C. Secondary goat anti-mouse (Dylight 488) overnight at 4C
Date published: 2018-11-27
Rated 4 out of 5 by from The fluorescence is not so nice in Western Blot. This antibody worked in the Western blot, but the fluorescence is not so nice. It seems to recognize only the cytosolic YAP.
Date published: 2018-05-17
Rated 5 out of 5 by from Good quality I used this antibody for IHC and the result is good.
Date published: 2018-04-25
Rated 5 out of 5 by from Great features I used this antibody on nuclear extraction of pancreatic cancer cell line and have good bands.
Date published: 2018-03-14
Rated 5 out of 5 by from 不错! sc-271134抗体在做斑马鱼5dpf胚胎免疫荧光染色的实验中,在胞浆和胞膜都能观察到信号,不错!
Date published: 2017-12-12
Rated 3 out of 5 by from YAP 抗体 (H-9) X ChIP实验 我们用这个抗体做ChIP实验,但是没有得到阳性结果,有可能该蛋白并不结合我们的目标区域,也可能是抗体问题,目前无法确认。
Date published: 2017-12-11
Rated 5 out of 5 by from Clean band at expected molecular weight (~65kDa) Sample: MDA-MB-231 wcl (30ug) Blocking: 1h in blocking buffer (0.05% tween-20 in TBS with 5% skin milk). Primary antibody: 1:200 in blocking buffer @ 4oC, overnight. Secondary antibody: 1:3000 in blocking buffer. Exposure time: 30 seconds.
Date published: 2017-11-18
Rated 5 out of 5 by from Works Great for IF We tried this antibody for immunofluorescence in CaCo and MDCK cells. It works very nicely! We used a 1:100 dilution.
Date published: 2017-10-06
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