Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
1. Kallet, R.H., et al. 2000. Am. J. Respir. Crit. Care Med. 161: 1149-1153. PMID: 10764304 2. Desmarais, W.T., et al. 2002. Structure. 10: 1063-1072. PMID: 12176384 3. Ghalanbor, Z., et al. 2008. Protein Pept. Lett. 15: 212-214. PMID: 18289113 4. Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY. CSHL Press.
Thank you for your question. This chemical, Tris base (CAS 77-86-1), Is not tested for Molecular biology grade. However, it is high quality and we suggest using Molecular biology grade solvents for filtering and testing a sample prior to use.
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