Tris base A high quality buffer used in the range of physiological pH 7-9

Tris base  (CAS 77-86-1)

Tris base | CAS 77-86-1 is rated 4.8 out of 5 by 5.
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| See product citations (1)
Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol
Application: A high quality buffer used in the range of physiological pH 7-9
CAS Number: 77-86-1
Purity: ≥99%
Molecular Weight: 121.14
Molecular Formula: C4H11NO3
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Tris base sc-3715 500 g $27.00
Tris base sc-3715A 1 kg $45.00
Tris base sc-3715B 5 kg $195.00
Tris base sc-3715C 10 kg $390.00

Tris base, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.


References

1. Kallet, R.H., et al. 2000. Am. J. Respir. Crit. Care Med. 161: 1149-1153. PMID: 10764304
2. Desmarais, W.T., et al. 2002. Structure. 10: 1063-1072. PMID: 12176384
3. Ghalanbor, Z., et al. 2008. Protein Pept. Lett. 15: 212-214. PMID: 18289113
4. Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY. CSHL Press.

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in water (550 mg/ml), ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), chloroform (0.05 mg/ml), and alkaline solution.
pH :
useful pH range: 7.0 - 9.0
Storage :
Store at room temperature
Melting Point :
168-172° C
Boiling Point :
219-220° C at 10 mmHg
Density :
1.35 g/cm3 at 20° C
Refractive Index :
n20D 1.54 (Predicted)
pK Values :
pKa: 8.2 (at 20° C), 8.06 (at 25° C), 7.72 (at 37° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
TY2900000
PubChem CID :
6503
Merck Index :
14: 9772
MDL Number :
MFCD00004679
EC Number :
201-064-4
Beilstein Registry :
741883
SMILES :
C(C(CO)(CO)N)O

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Certificate of Analysis

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Tris base Product Citations

See how others have used Tris base. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 12124396
Hansson, LO. et al. 2002. Two sequence motifs from HIF-1alpha bind to the DNA-binding site of p53. Proc. Natl. Acad. Sci. U.S.A.. 99: 10305-10309.

Citations 1 to 1 of 1 total

What is the country of origin?

Asked by: SCM4
Previous lots have originated in China.
Answered by: Tech Service 11
Date published: 2017-03-17

Is this compound molecular biology grade?

Asked by: SCchem16
Thank you for your question. This chemical, Tris base (CAS 77-86-1), Is not tested for Molecular biology grade. However, it is high quality and we suggest using Molecular biology grade solvents for filtering and testing a sample prior to use.
Answered by: Chemical Support 2
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice product It is much cheaper than Sigma. Strongly recommend it!
Date published: 2017-09-27
Rated 5 out of 5 by from Great value. We bought this month ago and we are perfectly happy with that.
Date published: 2017-03-23
Rated 5 out of 5 by from great quality and cheaper I bought this product and It is much cheaper compared to others!
Date published: 2016-11-29
Rated 5 out of 5 by from Our lab buys this in bulk to make buffers Our lab buys this in bulk to make buffers, good quality.
Date published: 2015-10-28
Rated 4 out of 5 by from I will rate this product a 4 and rate 5 I will rate this product a 4 and rate 5 for your logistics, customer service and support.
Date published: 2015-04-20
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