TCP-1 epsilon Antibody (G-3) recommended for detection of TCP-1 ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

TCP-1 ε Antibody (G-3): sc-376188

TCP-1 ε Antibody (G-3) is rated 5.0 out of 5 by 4.
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Datasheets
    • TCP-1 ε Antibody (G-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 111-390 mapping within an internal region of TCP-1 ε of human origin
    • recommended for detection of TCP-1 ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of TCP-1 ε (G-3): sc-376188.
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What is the recommended fixation method for immunofluorescence staining with TCP-1 ε (G-3): sc-376188 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from a fantastic antibody for WB This antibody recognizes a very clear and strong signal at the predicted M.W. (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-20
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-20
Rated 5 out of 5 by from Produced robust positive band in WB in F9 Produced robust positive band in WB in F9 whole cell lysate and human testis, mouse testis and rat testis tissue extracts. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Strong band of interest observed in WB in Caki Strong band of interest observed in WB in Caki-1 , HeLa and ES-2 whole cell lysates. -SCBT QC
Date published: 2015-04-05
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