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western blotting image from recommended Shh (E-1) monoclonal antibody replacement. Image 137799
This polyclonal antibody has been discontinued. See our recommended Shh (E-1) monoclonal antibody (sample data shown).
western blotting image from recommended Shh (E-1) monoclonal antibody replacement. Image 137799
This polyclonal antibody has been discontinued. See our recommended Shh (E-1) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended Shh (E-1) monoclonal antibody (sample data shown).

Shh Antibody (H-160): sc-9024

4.5(13)
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  • Shh Antibody (H-160) is a rabbit polyclonal IgG; 200 µg/ml
  • Discontinued polyclonal antibody

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Description

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For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

Shh (E-1): sc-365112 [ Recommended monoclonal replacement for Shh (H-160) ]

Product NameCatalog #UNITPriceQtyFAVORITES

Shh Antibody (E-1)

sc-365112
200 µg/ml
$316.00

Shh Antibody (E-1) AC

sc-365112 AC
500 µg/ml, 25% agarose
$416.00

Shh Antibody (E-1) HRP

sc-365112 HRP
200 µg/ml
$316.00

Shh Antibody (E-1) FITC

sc-365112 FITC
200 µg/ml
$330.00

Shh Antibody (E-1) PE

sc-365112 PE
200 µg/ml
$343.00

Shh Antibody (E-1) Alexa Fluor® 488

sc-365112 AF488
200 µg/ml
$357.00

Shh Antibody (E-1) Alexa Fluor® 546

sc-365112 AF546
200 µg/ml
$357.00

Shh Antibody (E-1) Alexa Fluor® 594

sc-365112 AF594
200 µg/ml
$357.00

Shh Antibody (E-1) Alexa Fluor® 647

sc-365112 AF647
200 µg/ml
$357.00

Shh Antibody (E-1) Alexa Fluor® 680

sc-365112 AF680
200 µg/ml
$357.00

Shh Antibody (E-1) Alexa Fluor® 790

sc-365112 AF790
200 µg/ml
$357.00

Shh (E-1) Neutralizing Peptide

sc-365112 P
100 µg/0.5 ml
$68.00

Would this (discontinued) polyclonal antibody be expected to detect Indian Hedgehog (Ihh)?  The description stated it detected "broad range of hedgehog family proteins of mouse, rat, human.."

Asked by: David F
Thank you for your question. Yes, this antigen shares significant sequence identity (>90%) with Ihh.
Answered by: Technical Support
Date published: 2019-05-17

Hi, one comment - this H-160 pAb is useful for mouse tissue IF or IHC. Your mAb would be less useful for the same purpose. Where could I find the antigen region information that was used to raise the H-160 pAb?

Asked by: kurakura
This now discontinued rabbit polyclonal antibody, Shh (H-160): sc-9024, was raised against a recombinant protein corresponding to amino acids 41-200 of Shh of human origin. Our recommended replacement mouse monoclonal antibody, Shh (E-1): sc-365112, is also suitable for IF and IHC(P) on mouse tissue. We offer the antibody directly conjugated to HRP, or a variety of fluorescent dyes and you could use the conjugated antibody for staining without having to probe using any anti-mouse IgG secondary antibody for detection. There are also methods for mouse on mouse staining using the unconjugated primary antibody. Please contact Technical Service if you have further questions.
Answered by: Technical Support
Date published: 2018-12-21
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Rated 5 out of 5 by from Shh Antibody (H-160) in WBThis antibody worked perfectly in WB both for human cervical cancer cell lines and immortalized human keratinocytes (HaCaT). It has shown differential expression among different cell lines and the expression was in line with the results of semi-quantitative RT-PCR.
Date published: 2021-04-14
Rated 5 out of 5 by from sc-9024I found the sc-9024 antibody very useful for immunohistochemistry, western blotting and immuno fluorescence confocal microscopy experiments. Thank you
Date published: 2019-10-03
Rated 5 out of 5 by from Works well for Western blot and IHCWorks well for Western blot and IHC-P
Date published: 2015-12-18
Rated 5 out of 5 by from Works well for IHC on FFPE human pancreaticWorks well for IHC on FFPE human pancreatic adenocarcinoma sections.
Date published: 2015-12-14
Rated 4 out of 5 by from Published data using antibody with paraffinPublished data using antibody with paraffin embedded sections.
Date published: 2015-11-12
Rated 4 out of 5 by from Various publishable data in IHC using mouse embryoVarious publishable data in IHC using mouse embryo, bladder epithelial, ovary, testes and duodena. -SCBT Publication Review
Date published: 2015-04-14
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Great AntibodyGreat Antibody! Do not have any complaints as it has been continuously performing well.
Date published: 2015-03-30
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Shh Antibody (H-160) is rated 4.5 out of 5 by 13.
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