Potassium phosphate tribasic A reagent with very high buffering capacity widely used in molecular biology

Potassium phosphate tribasic (CAS 7778-53-2)

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Synonym: Tripotassium phosphate
Application: A reagent with very high buffering capacity widely used in molecular biology
CAS Number: 7778-53-2
Purity: ≥97%
Molecular Weight: 212.27
Molecular Formula: K3PO4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Potassium phosphate tribasic is a reagent with a very high buffering capacity. It is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Neutral potassium phosphate buffer solutions may be prepared with a mixture of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium Phosphate buffers are very useful in numerous applications, but with the following limitations; precipitation of Ca2+ and Mg2+, inhibition of restriction enzyme activity, and interference in DNA ligation or bacterial transformation protocols. Has been used to study the effects of freezing and thawing on the stability of proteins sensitive to conformational changes; it was found that potassium phosphate buffers offered improved pH stability as opposed to sodium phosphate buffers. Potassium Phosphate has also been used for the extraction of keratohyalin protein from bovine tissue.

Additional forms available:
Potassium Phosphate, Dibasic, Anhydrous (sc-203210)
Potassium Phosphate, Monobasic (sc-203211)
Potassium phosphate tribasic monohydrate (sc-250767)
Potassium phosphate dibasic solution (sc-301600)
Potassium Phosphate Monobasic solution (sc-301601)


References

1. Ugel, A.R. and Idler, W. 1972. J. Cell Biol. 52: 453-464. PMID: 5057980
2. Pikal-Cleland, K.A., et al. 2000. Arch. Biochem. Biophys. 384: 398-406. PMID: 11368330

Appearance :
Powder or granules
Physical State :
Solid
Solubility :
Soluble in water (100 mg/mL). Insoluble in alcohol.
Storage :
Store at room temperature
Melting Point :
1340° C
Density :
2.56 g/cm3 at 25° C (lit.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
TC8450000
PubChem CID :
62657
Merck Index :
14: 7660
MDL Number :
MFCD00036295
EC Number :
231-907-1
SMILES :
[O-]P(=O)([O-])[O-].[K+].[K+].[K+]

Download SDS (MSDS)

Certificate of Analysis

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