p53 Antibody (DO-1)
  • p53 Antibody (DO-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
  • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
  • recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of human origin by WB, IP, IF, IHC(P) and FCM
  • available conjugated to agarose (sc-126 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-126 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to biotin (sc-126 B), 200 µg/ml for WB, IHC(P) and ELISA; TRITC (sc-126 TRITC, 200 µg/ml), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5), Alexa Fluor® 405 (sc-126 AF405), 100 tests in 2 ml, for IF, IHC(P) and FCM
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

p53 Antibody (DO-1): sc-126

p53 Antibody (DO-1) is rated NaN out of 5 by 60.
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Datasheets
    • p53 Antibody (DO-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
    • recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose (sc-126 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-126 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to biotin (sc-126 B), 200 µg/ml for WB, IHC(P) and ELISA; TRITC (sc-126 TRITC, 200 µg/ml), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5), Alexa Fluor® 405 (sc-126 AF405), 100 tests in 2 ml, for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
p53 Antibody (DO-1) sc-126 200 µg/ml $279.00
p53 Antibody (DO-1) AC sc-126 AC 500 µg/ml, 25% ag $366.00
p53 Antibody (DO-1) Alexa Fluor® 405 sc-126 AF405 100 µg/2 ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 488 sc-126 AF488 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 647 sc-126 AF647 200 µg/ml $314.00
p53 Antibody (DO-1) B sc-126 B 200 µg/ml $282.00
p53 Antibody (DO-1) FITC sc-126 FITC 200 µg/ml $292.00
p53 Antibody (DO-1) HRP sc-126 HRP 200 µg/ml $279.00
p53 Antibody (DO-1) PCPC5 sc-126 PCPC5 100 tests in 2 ml $310.00
p53 Antibody (DO-1) PE sc-126 PE 200 µg/ml $303.00
p53 Antibody (DO-1) PerCP sc-126 PerCP 100 tests in 2 ml $310.00
p53 Antibody (DO-1) TRITC sc-126 TRITC 200 µg/ml $282.00
p53 Antibody (DO-1) X sc-126 X 200 µg/0.1 ml $279.00

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p53 Antibody (DO-1) Product Citations

See how others have used p53 Antibody (DO-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1426 total

PMID: # 28098348
Squillaro, T. et al. 2017. Impact of lysosomal storage disorders on biology of mesenchymal stem cells: Evidences from in vitro silencing of glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes. J. Cell. Physiol..

PMID: # 28154167
Ohoka, N. et al. 2017. In Vivo Knockdown of Pathogenic Proteins via Specific and Nongenetic IAP-dependent Protein Erasers (SNIPERs). J. Biol. Chem..

PMID: # 28073943
Castellini, L. et al. 2017. KDM4B/JMJD2B is a p53 target gene that modulates the amplitude of p53 response after DNA damage. Nucleic Acids Res..

PMID: # 28035772
Zubiete-Franco, I. et al. 2017. Deregulated neddylation in liver fibrosis. Hepatology (Baltimore, Md.). 65: 694-709.

PMID: # 28059478
Tamilzhalagan, S. et al. 2017. Amplified 7q21-22 gene MCM7 and its intronic miR-25 suppress COL1A2 associated genes to sustain intestinal gastric cancer features. Mol. Carcinog..

PMID: # 27341992
Monteith, JA. et al. 2016. A rare DNA contact mutation in cancer confers p53 gain-of-function and tumor cell survival via TNFAIP8 induction. Mol Oncol. 10: 1207-20.

PMID: # 26725939
Han, MA. et al. 2016. Galangin sensitizes TRAIL-induced apoptosis through down-regulation of anti-apoptotic proteins in renal carcinoma Caki cells. Sci Rep. 6: 18642.

PMID: # 26784393
Chu, X. et al. 2016. PTD-fused p53 as a potential antiviral agent directly suppresses HBV transcription and expression. Antiviral Res.. 127: 41-9.

PMID: # 26935151
Lee, SJ. et al. 2016. Rosa hybrida extract suppresses vascular smooth muscle cell responses by the targeting of signaling pathways, cell cycle regulation and matrix metalloproteinase-9 expression. International journal of molecular medicine. 37: 1119-26.

PMID: # 26994140
Cui, H. et al. 2016. The Stress-responsive Gene ATF3 Mediates Dichotomous UV Responses by Regulating the Tip60 and p53 Proteins. J. Biol. Chem.. 291: 10847-57.

Citations 1 to 10 of 1426 total

I used this antibody for WB to detect endogenous p53 in several cell lines. However, non specific bands were detected around 50-55kDa. My question is what blocking reagents and conditions are suitable to minimize the non specific band  ? 

Asked by: Shin T
Thank you for your question. We recommend using 5% nonfat milk in TBST (TBS Blotto A: sc-2333) for blocking and antibody incubations. We would recommend running a secondary antibody control, where you incubate your membrane without primary antibody, to see if the non-specific bands are a result of your secondary antibody binding non-specifically. If that is the case, which is fairly common, then as an alternative we would recommend using our Mouse IgG kappa binding protein (m-IgGκ BP-HRP: sc-516102) conjugated to HRP in place of a conventional secondary antibody. A link to a description of this product is provided below. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Below is a link to our recommended protocol for Western blotting with this antibody. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support
Date published: 2017-02-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service
Date published: 2016-12-13

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody (enter name here) is currently available conjugated to agarose (sc-126 AC), HRP (sc-126 HRP), phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), biotin (sc-126 B), TRITC (sc-126 TRITC), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5) and Alexa Fluor® 405 (sc-126 AF405).
Answered by: Tech Service
Date published: 2016-12-13

what is the meaning of DO-1

Asked by: naser49
Thank you for your question. This is a mouse monoclonal antibody against p53, catalog number is sc-126, and (DO-1) is the clone name for this specific mouse monoclonal antibody. The clone name is a unique identifier assigned to monoclonal antibodies that are separate from the catalog number.
Answered by: Technical Support
Date published: 2016-09-29
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Rated 5 out of 5 by from good Mab I bought the p53 mouse clonal antibody. Works good in Western at dilution 1:500,clear band.
Date published: 2017-02-23
Rated 5 out of 5 by from Excellent antibody I requested this antibody as a sample. It recognizes both mutant and WT p53 from human the pancreatic cancer cells that I tested on. An excellent choice for anyone who would be looking for a clean antibody againt p53.
Date published: 2017-02-22
Rated 4 out of 5 by from Nicht erwartete Ergebnisse Sehr geehrtes Santa Cruz Team, ihr habt diesen Monoklonalen AK als Alternative zum Polyklonalen SC-6243 angeboten. Leider zielt er auf das N Terminale Ende, wohingegen meine Mutante bei 15 kDa mit dem alten AK erkennbar, am C Terminalen Ende detektiert werden kann. Die wildtyp P53 Bande war allerdings in Ordnung.
Date published: 2017-02-15
Rated 5 out of 5 by from Excellent Very great for WB with a dilution factor of 1:1,000.
Date published: 2017-02-12
Rated 5 out of 5 by from good sample antibody For WB detect of P53 , high recommend 1:600 dilution use TBST dilution buffer.
Date published: 2017-02-11
Rated 5 out of 5 by from Great IHC data, Great IHC data, cytoplasmic staining in paraffin-embedded human skeletal muscle tissue.It showed very good perfomance,Dilution ratio 1:200
Date published: 2017-02-08
Rated 5 out of 5 by from Good antibody This antibody worked great in my WB, we have purchased this antibody several times, we have not encountered any problems in any applications.
Date published: 2017-02-07
Rated 5 out of 5 by from The product is good. Superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC.The antibody is good and strong. But there are few non light non specific bands too !!
Date published: 2017-02-06
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