p53 Antibody (DO-1)
  • p53 Antibody (DO-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
  • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
  • recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of human origin by WB, IP, IF, IHC(P) and FCM
  • available conjugated to agarose (sc-126 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-126 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to biotin (sc-126 B), 200 µg/ml for WB, IHC(P) and ELISA; TRITC (sc-126 TRITC, 200 µg/ml), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5), Alexa Fluor® 405 (sc-126 AF405), 100 tests in 2 ml, for IF, IHC(P) and FCM
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

p53 Antibody (DO-1): sc-126

p53 Antibody (DO-1) is rated 4.8 out of 5 by 76.
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Datasheets
    • p53 Antibody (DO-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
    • recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose (sc-126 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-126 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to biotin (sc-126 B), 200 µg/ml for WB, IHC(P) and ELISA; TRITC (sc-126 TRITC, 200 µg/ml), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5), Alexa Fluor® 405 (sc-126 AF405), 100 tests in 2 ml, for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
p53 Antibody (DO-1) sc-126 200 µg/ml $279.00
p53 Antibody (DO-1) AC sc-126 AC 500 µg/ml, 25% ag $366.00
p53 Antibody (DO-1) Alexa Fluor® 405 sc-126 AF405 100 µg/2 ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 488 sc-126 AF488 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 647 sc-126 AF647 200 µg/ml $314.00
p53 Antibody (DO-1) B sc-126 B 200 µg/ml $282.00
p53 Antibody (DO-1) FITC sc-126 FITC 200 µg/ml $292.00
p53 Antibody (DO-1) HRP sc-126 HRP 200 µg/ml $279.00
p53 Antibody (DO-1) PCPC5 sc-126 PCPC5 100 tests in 2 ml $310.00
p53 Antibody (DO-1) PE sc-126 PE 200 µg/ml $303.00
p53 Antibody (DO-1) PerCP sc-126 PerCP 100 tests in 2 ml $310.00
p53 Antibody (DO-1) TRITC sc-126 TRITC 200 µg/ml $282.00
p53 Antibody (DO-1) X sc-126 X 200 µg/0.1 ml $279.00

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p53 Antibody (DO-1) Product Citations

See how others have used p53 Antibody (DO-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1532 total

PMID: # 28222755
Singh, VP. et al. 2017. WD-repeat protein WDR13 is a novel transcriptional regulator of c-Jun and modulates intestinal homeostasis in mice. BMC Cancer. 17: 148.

PMID: # 28256619
Riddick, G. et al. 2017. A Core Regulatory Circuit in Glioblastoma Stem Cells Links MAPK Activation to a Transcriptional Program of Neural Stem Cell Identity. Sci Rep. 7: 43605.

PMID: # 27927016
Seo, BR. et al. 2017. Inhibition of Cathepsin S Induces Mitochondrial ROS That Sensitizes TRAIL-Mediated Apoptosis Through p53-Mediated Downregulation of Bcl-2 and c-FLIP. Antioxid. Redox Signal..

PMID: # 28098348
Squillaro, T. et al. 2017. Impact of lysosomal storage disorders on biology of mesenchymal stem cells: Evidences from in vitro silencing of glucocerebrosidase (GBA) and alpha-galactosidase A (GLA) enzymes. J. Cell. Physiol..

PMID: # 27935327
Chhabra, A. et al. 2017. Activation induced cell death (AICD) of human melanoma antigen-specific TCR engineered CD8 T cells involves JNK, Bim and p53. Expert Opin. Ther. Targets. 21: 117-129.

PMID: # 28154167
Ohoka, N. et al. 2017. In Vivo Knockdown of Pathogenic Proteins via Specific and Nongenetic IAP-dependent Protein Erasers (SNIPERs). J. Biol. Chem..

PMID: # 27889610
Fukuda, T. et al. 2017. CACUL1/CAC1 attenuates p53 activity through PML post-translational modification. Biochem. Biophys. Res. Commun.. 482: 863-869.

PMID: # 28092675
Worrall, C. et al. 2017. Unbalancing p53/Mdm2/IGF-1R axis by Mdm2 activation restrains the IGF-1-dependent invasive phenotype of skin melanoma. Oncogene.

PMID: # 28176872
Prakash Bangalore, M. et al. 2017. Genotoxic Effects of Culture Media on Human Pluripotent Stem Cells. Sci Rep. 7: 42222.

PMID: # 28073943
Castellini, L. et al. 2017. KDM4B/JMJD2B is a p53 target gene that modulates the amplitude of p53 response after DNA damage. Nucleic Acids Res..

Citations 1 to 10 of 1532 total

Hi, I noticed that the p53 DO-1 antibody on the abcam website (ab1101) was said 'Does not react with: Mouse, Rat'. But your product datasheet said it can react with p53 from mouse, rat and human origin. Just wondering if this has been tested in house?

Asked by: Sue8080
Thank you for your question. This mouse monoclonal antibody, p53 (DO-1): sc-126, is a well established clone that is primarily used for detection of p53 of human origin. We are aware of numerous product citations using the DO-1 clone in mouse samples, and we recently obtained some Western blot data in-house in NIH/3T3 whole cell lysate. However, upon closer review this Western blot data will be removed. And we are going to change the species reactivity back to recommended for human only.
Answered by: Technical Support
Date published: 2017-05-15

cual es la secuencia de aminoacidos que reconoce?

Asked by: karenAna
P53 (DO-1): se levantó sc-126 frente a una secuencia entre los aminoácidos 11 - 25 de p53 de origen humano (adhesión nº P04637). p53 (DO-1): sc-126 recognizes an N-terminal epitope mapping between amino acid residues 11 - 25 of p53 of human origin (accession# P04637).
Answered by: Tech Service 3
Date published: 2017-03-13

I used this antibody for WB to detect endogenous p53 in several cell lines. However, non specific bands were detected around 50-55kDa. My question is what blocking reagents and conditions are suitable to minimize the non specific band  ? 

Asked by: Shin T
Thank you for your question. We recommend using 5% nonfat milk in TBST (TBS Blotto A: sc-2333) for blocking and antibody incubations. We would recommend running a secondary antibody control, where you incubate your membrane without primary antibody, to see if the non-specific bands are a result of your secondary antibody binding non-specifically. If that is the case, which is fairly common, then as an alternative we would recommend using our Mouse IgG kappa binding protein (m-IgGκ BP-HRP: sc-516102) conjugated to HRP in place of a conventional secondary antibody. A link to a description of this product is provided below. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Below is a link to our recommended protocol for Western blotting with this antibody. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support
Date published: 2017-02-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service
Date published: 2016-12-13

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody (enter name here) is currently available conjugated to agarose (sc-126 AC), HRP (sc-126 HRP), phycoerythrin (sc-126 PE), fluorescein (sc-126 FITC), Alexa Fluor® 488 (sc-126 AF488) or Alexa Fluor® 647 (sc-126 AF647), biotin (sc-126 B), TRITC (sc-126 TRITC), PerCP (sc-126 PerCP), PerCP-Cy5.5 (sc-126 PCPC5) and Alexa Fluor® 405 (sc-126 AF405).
Answered by: Tech Service
Date published: 2016-12-13

what is the meaning of DO-1

Asked by: naser49
Thank you for your question. This is a mouse monoclonal antibody against p53, catalog number is sc-126, and (DO-1) is the clone name for this specific mouse monoclonal antibody. The clone name is a unique identifier assigned to monoclonal antibodies that are separate from the catalog number.
Answered by: Technical Support
Date published: 2016-09-29
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Rated 5 out of 5 by from western blot Clear western blot signal background was obtained at 1:500 dilution.
Date published: 2017-05-13
Rated 5 out of 5 by from Western blot Used for Western blot of mouse brain sample. Worked good and got specific band at 53kda.
Date published: 2017-05-13
Rated 5 out of 5 by from Great This antibody works well in western blotting. The band is strong and specific.
Date published: 2017-05-12
Rated 4 out of 5 by from Buono per campioni umani Ho utilizzato questo anticorpo per WB (diluizione 1:500) su campioni umani e murini (HEK e MEF). In base alla mia esperienza posso dire che è un anticorpo efficiente nel riconoscere la proteina p53 in campioni umani ma non murini.
Date published: 2017-05-11
Rated 5 out of 5 by from clean band Good antibody for WB using 1:500 dilution in A-431 cells, I strongly recommend
Date published: 2017-05-10
Rated 5 out of 5 by from p53 antibody is great I used 1/200 overnite in milk. worked great in human cancer cell lysates
Date published: 2017-05-01
Rated 5 out of 5 by from Good IHC antibody Analysis human breast carcinoma tissue showing nuclear staining by IHC, very excellent antibody
Date published: 2017-04-30
Rated 5 out of 5 by from Very widly used It works well in human samples by WB, very specific and very little background
Date published: 2017-04-17
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