p53 Antibody (DO-1) recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

p53 Antibody (DO-1): sc-126

p53 Antibody (DO-1) is rated 4.7 out of 5 by 148.
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Datasheets
    • p53 Antibody (DO-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
    • recommended for detection of wild type and mutant p53 under denaturing and non-denaturing conditions of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-126 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of p53 (DO-1): sc-126.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
p53 Antibody (DO-1) sc-126 200 µg/ml $279.00
p53 Antibody (DO-1) X sc-126 X 200 µg/0.1 ml $279.00
p53 Antibody (DO-1) AC sc-126 AC 500 µg/ml, 25% agarose $366.00
p53 Antibody (DO-1) HRP sc-126 HRP 200 µg/ml $279.00
p53 Antibody (DO-1) FITC sc-126 FITC 200 µg/ml $292.00
p53 Antibody (DO-1) PE sc-126 PE 200 µg/ml $303.00
p53 Antibody (DO-1) Alexa Fluor® 488 sc-126 AF488 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 546 sc-126 AF546 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 594 sc-126 AF594 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 647 sc-126 AF647 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 680 sc-126 AF680 200 µg/ml $314.00
p53 Antibody (DO-1) Alexa Fluor® 790 sc-126 AF790 200 µg/ml $314.00
p53 Antibody (DO-1) B sc-126 B 200 µg/ml $282.00
p53 Antibody (DO-1) TRITC sc-126 TRITC 200 µg/ml $282.00
p53 Antibody (DO-1) Alexa Fluor® 405 sc-126 AF405 100 µg/2 ml $314.00
p53 Antibody (DO-1) PerCP sc-126 PerCP 100 tests in 2 ml $310.00
p53 Antibody (DO-1) PerCP-Cy5.5 sc-126 PCPC5 100 tests in 2 ml $310.00

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p53 Antibody (DO-1)Product Citations

See how others have used p53 Antibody (DO-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 3734 total

PMID: # 29895829  Lee, Y. et al. 2018. Nat Commun. 9: 2301.

PMID: # 29599136  Adamson, SE. et al. 2018. Arterioscler. Thromb. Vasc. Biol. 38: 1020-1029.

PMID: # 29769639  Li, X. et al. 2018. Cell Death Differ.

PMID: # 29879550  Plafker, KS. et al. 2018. Redox Biol. 17: 411-422.

PMID: # 29844578  Li, Z. et al. 2018. Cell Res.

PMID: # 29953728  Maan, M. et al. 2018. FEBS J.

PMID: # 29883784  Pereira, JM. et al. 2018. Food Chem. Toxicol.

PMID: # 29622769  Kim, JH. et al. 2018. Exp. Mol. Med. 50: 3.

PMID: # 29512682  Feng, C. et al. 2018. Int. J. Mol. Med. 41: 3316-3326.

PMID: # 29545174  Ceballos, MP. et al. 2018. Toxicol. Lett. 289: 63-74.

Citations 1 to 10 of 3734 total

怎么获得免费小样

Asked by: guoqinyu
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

Hi, I was wondering if you also sell the peptide matching to this antibody, for elution following immunoprecipitation? Many thanks in advance.

Asked by: Moli
Thank you for your question. We do not have a blocking peptide available for this antibody, p53 (DO-1): sc-126.
Answered by: Technical Support
Date published: 2017-10-05

Hi, just wonder if this antibody will be able to detect cell lines with p53 mutants (all located from exon 5-8) ? Thank you.

Asked by: Rickkkk
Thank you for your question. This mouse monoclonal antibody, p53 (DO-1): sc-126, is recommended for detection of wild type and mutant p53 of human origin by WB, IP, IF, IHC(P) and FCM.
Answered by: Technical Support
Date published: 2018-07-27

whether these antibodies were specific to drosophila.

Asked by: USHA BHOCAL
Thank you for your question. We do not recommend this antibody, p53 (DO-1): sc-126, for detection of p53 in drosophila. This antibody is recommended for detection of human p53. We recommend using either our p53 (E-5): sc-74573, or our p53 (C-11): sc-74574, which are specific for drosophila.
Answered by: Technical Support
Date published: 2017-07-31

I want to use this antibody for IHC on FFPE human brain samples, but I don't find the preferred antigen retrieval method (citrate or EDTA?)

Asked by: bjonny
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody, p53 (DO-1): sc-126. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-06

Hi, I noticed that the p53 DO-1 antibody on the abcam website (ab1101) was said 'Does not react with: Mouse, Rat'. But your product datasheet said it can react with p53 from mouse, rat and human origin. Just wondering if this has been tested in house?

Asked by: Sue8080
Thank you for your question. This mouse monoclonal antibody, p53 (DO-1): sc-126, is a well established clone that is primarily used for detection of p53 of human origin. We are aware of numerous product citations using the DO-1 clone in mouse samples, and we recently obtained some Western blot data in-house in NIH/3T3 whole cell lysate. However, upon closer review this Western blot data will be removed. And we are going to change the species reactivity back to recommended for human only.
Answered by: Technical Support
Date published: 2017-06-26

cual es la secuencia de aminoacidos que reconoce?

Asked by: karenAna
P53 (DO-1): se levantó sc-126 frente a una secuencia entre los aminoácidos 11 - 25 de p53 de origen humano (adhesión nº P04637). p53 (DO-1): sc-126 recognizes an N-terminal epitope mapping between amino acid residues 11 - 25 of p53 of human origin (accession# P04637).
Answered by: Tech Service 3
Date published: 2018-10-10

I used this antibody for WB to detect endogenous p53 in several cell lines. However, non specific bands were detected around 50-55kDa. My question is what blocking reagents and conditions are suitable to minimize the non specific band  ? 

Asked by: Shin T
Thank you for your question. We recommend using 5% nonfat milk in TBST (TBS Blotto A: sc-2333) for blocking and antibody incubations. We would recommend running a secondary antibody control, where you incubate your membrane without primary antibody, to see if the non-specific bands are a result of your secondary antibody binding non-specifically. If that is the case, which is fairly common, then as an alternative we would recommend using our Mouse IgG kappa binding protein (m-IgGκ BP-HRP: sc-516102) conjugated to HRP in place of a conventional secondary antibody. A link to a description of this product is provided below. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Below is a link to our recommended protocol for Western blotting with this antibody. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support
Date published: 2017-02-16
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Rated 5 out of 5 by from good product This antibody works pretty well for our western blot experiment.
Date published: 2018-08-22
Rated 4 out of 5 by from p53 antibody for western Received a free sample to test with our cells...worked great. Will order soon
Date published: 2018-08-21
Rated 1 out of 5 by from Did not work for WB analysis Tried at a 1:500 and 1:1000 dilution in human bladder cancer cells with wild type and mutant p53 but detected no signal.
Date published: 2018-06-19
Rated 5 out of 5 by from great work We uploaded 20ug total protein from induced HCT116 cell and detected a very clear band.
Date published: 2018-06-12
Rated 5 out of 5 by from staisfied result The result is staisfied when diluted 1:300 by WB.Great bands at 53kDa.
Date published: 2018-05-26
Rated 5 out of 5 by from Excellent antibody Very clean and specific antibody for human breast cancer cells as well as Cos7 cells
Date published: 2018-04-23
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from 效果Perfect 近期刚使用了这支抗体,按照说明书给的稀释浓度选择5%BSA液按照1:1000的稀释比例稀释,发出的条带整体很nice。
Date published: 2018-04-21
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