NFAT5 Antibody (F-9): sc-398171

NFAT5 Antibody (F-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects NFAT5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NFAT5 (F-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NFAT5 plays a crucial role in cellular responses to osmotic stress and is essential for maintaining cellular homeostasis, particularly in kidney cells. NFAT5 is localized primarily in the nucleus, where NFAT5 regulates the expression of genes involved in osmotic regulation and inflammation. NFAT5′s ability to translocate to the nucleus in response to changes in osmotic pressure highlights NFAT5′s importance in cellular adaptation to environmental stressors. NFAT5 interacts with other proteins, such as the transcription factor AP-1, to form complexes that modulate gene expression, thereby influencing various physiological processes. NFAT5 activation is mediated by increases in intracellular calcium levels, which trigger NFAT5 dephosphorylation by calcineurin, leading to a conformational change that exposes NFAT5′s nuclear localization signal. This process enables NFAT5 translocation from the cytosol to the nucleus, allowing NFAT5 to exert regulatory functions effectively.

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NFAT5 Antibody (F-9) References:

1. Recognition of NFATp/AP-1 composite elements within genes induced upon the activation of immune cells.  |  Kel, A., et al. 1999. J Mol Biol. 288: 353-76. PMID: 10329147
2. NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun.  |  Lopez-Rodríguez, C., et al. 1999. Proc Natl Acad Sci U S A. 96: 7214-9. PMID: 10377394
3. Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells.  |  Ju, J., et al. 2007. Cell Res. 17: 264-70. PMID: 17310218
4. NFAT5 promotes arteriogenesis via MCP-1-dependent monocyte recruitment.  |  Lin, XC., et al. 2020. J Cell Mol Med. 24: 2052-2063. PMID: 31883300
5. The role of the osmosensitive transcription factor NFAT5 in corneal edema resorption after injury.  |  Hadrian, K., et al. 2023. Exp Mol Med. 55: 565-573. PMID: 36869067
6. Characterization of gene expression in the kidney of renal tubular cell-specific NFAT5 knockout mice.  |  Ono, M., et al. 2024. Am J Physiol Renal Physiol. 326: F394-F410. PMID: 38153851
7. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins.  |  Hoey, T., et al. 1995. Immunity. 2: 461-72. PMID: 7749981
8. Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc.  |  Park, J., et al. 1996. J Biol Chem. 271: 20914-21. PMID: 8702849
9. Expression of NFAT-family proteins in normal human T cells.  |  Lyakh, L., et al. 1997. Mol Cell Biol. 17: 2475-84. PMID: 9111316
10. Transcription factors of the NFAT family: regulation and function.  |  Rao, A., et al. 1997. Annu Rev Immunol. 15: 707-47. PMID: 9143705
11. Distinct NFAT family proteins are involved in the nuclear NFAT-DNA binding complexes from human thymocyte subsets.  |  Amasaki, Y., et al. 1998. J Immunol. 160: 2324-33. PMID: 9498773
12. The transcription factor NF-ATc is essential for cardiac valve formation.  |  Ranger, AM., et al. 1998. Nature. 392: 186-90. PMID: 9515964

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFAT5 Antibody (F-9) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-NFAT5 Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFAT5 Antibody (F-9) Specifications

Alternate Product Name: NFAT5

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 67-300

Epitope Target: NFAT5

Epitope Species: human

Product Reactivity/Detection: NFAT5a, NFAT5b, NFAT5c, NFAT5z1 and NFAT5z2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for NFAT5 Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent