HEPBS (CAS 161308-36-7)

HEPBS, or 2-(Hydroxyethyl)piperazine-N′-2′-ethanesulfonic acid, is a zwitterionic biological buffer used extensively in biochemical and molecular biology research to maintain pH stability within physiological ranges. Its unique structure incorporates both a piperazine ring and an ethanesulfonic acid moiety, which contribute to its buffering capacity and solubility. HEPBS is particularly valued for its minimal metal ion binding, which is crucial in experiments sensitive to metal chelation effects. Unlike many traditional buffers, HEPBS operates effectively at near-physiological pH, typically between 6.8 and 8.2, making it ideal for maintaining the environment required for enzyme reactions and cellular cultures without perturbing the natural ionic balance. Its mechanism of action lies in its ability to release or absorb protons in response to changes in the pH of the solution, thereby stabilizing the pH by preventing drastic shifts. HEPBS has been instrumental in research environments, particularly in studies involving protein-ligand interactions, where maintaining a consistent pH is essential to avoid altering the structure and function of the proteins under study. Additionally, it has been used in the preparation of media for biological assays and as a buffering agent in electrophoresis and other biochemical analytical methods, showcasing its versatility and critical role in scientific investigations.