GATA4 Antibody (G-4): sc-25310

GATA-4 Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects GATA-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GATA-4 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GATA-4 is a critical transcription factor that plays a vital role in cardiac development and function, as GATA-4 is essential for regulating genes involved in heart formation and differentiation. GATA-4 is primarily located in the nucleus, where GATA-4 binds to specific DNA sequences to activate or repress target genes, thereby influencing cardiac tissue development. This nuclear localization enables GATA-4 to interact with other transcription factors and co-regulators, facilitating complex gene expression programs necessary for proper heart development. GATA-4 undergoes post-translational modifications, such as phosphorylation, which can modulate activity and stability, further underscoring GATA-4′s importance in cardiac biology. GATA-4′s ability to form complexes with other proteins, such as GATA-6 and various co-factors, enhances regulatory capacity, making GATA-4 a key player in transcriptional networks that govern heart development and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GATA4 Antibody (G-4) References:

1. Human transcription factor GATA-2. Evidence for regulation of preproendothelin-1 gene expression in endothelial cells.  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
2. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer.  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
3. GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut.  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
4. DNA-binding specificities of the GATA transcription factor family.  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
5. The human GATA-6 gene: structure, chromosomal location, and regulation of expression by tissue-specific and mitogen-responsive signals.  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GATA4 Antibody (G-4) has 323 citations in a variety of scientific publications.

Primary antibodies like Anti-GATA4 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GATA4 Antibody (G-4) Specifications

Alternate Product Name: GATA4

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 328-439

Epitope Target: GATA-4

Epitope Species: human

Product Reactivity/Detection: GATA-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 323 citations in various scientific publications

Available Conjugates/Forms for GATA4 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-GATA4 antibody is p-GATA4 Antibody (H-4): sc-377543.