Fast Green FCF A widely utilized tissue and protein stain used in IEF & SDS-PAGE, fluoresces near IR

Fast Green FCF (CAS 2353-45-9)

Fast Green FCF | CAS 2353-45-9 is rated 5.0 out of 5 by 1.
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Synonym: Solid Green FCF; Food Green No. 3
Application: A widely utilized tissue and protein stain used in IEF & SDS-PAGE, fluoresces near IR
CAS Number: 2353-45-9
Molecular Weight: 808.85
Molecular Formula: C37H34N2O10S3Na2
* Refer to Certificate of Analysis for lot specific data (including water content).
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Fast Green FCF has been a widely utilized tissue and protein stain used in IEF and SDS-PAGE. Has the ability to determine efficiency of transfer and localization of sample proteins on blotting membranes. Other applications of Fast Green FCF include using its clastogenic properties and it may also act at a presynaptic locus inhibiting neurotransmitter release in whole-cell voltage clamped hippocampal interneurons. Has been shown to be a beneficial stain to detail integumental injuries in Chinook salmon. When bound to protein, Fast Green FCF fluoresces near infrared; color index #42053.


References

1. Misra, R.N. and Misra, B. 1986. Mutat. Res. 170: 75-78. PMID: 3960045
2. Shen, H.D., et al. 1990. Electrophoresis. 11: 878-882. PMID: 2079030
3. van Hooft, J.A., 2002. Neurosci. Lett. 318: 163-165. PMID: 11803124
4. Luo, S., et al. 2006. Anal. Biochem. 350: 233-238. PMID: 16336940
5. Elliott, D.G., et al. 2009. Dis. Aquat. Org. 84: 139-150. PMID: 19476284

Appearance :
Powder or granules
Physical State :
Solid
Solubility :
Soluble in water (150 g/l) at 25° C, glycerin, glycol, alcohol, and ethanol.
Storage :
Store at room temperature
Melting Point :
290° C (lit.)(dec.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
BQ4425000
PubChem CID :
16887
Merck Index :
14: 3941
MDL Number :
MFCD00013053
EC Number :
219-091-5
Beilstein Registry :
5718212
SMILES :
CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=C(C=C(C=C5)O)S(=O)(=O)[O-].[Na+].[Na+]

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Fast Green FCF  Product Citations

See how others have used Fast Green FCF. Click on the entry to view the PubMed entry .

Citations 1 to 6 of 6 total

PMID: # 16732193  Wu MJ et al. 2006. Kidney Int. 69: 2029-2036.

PMID: # 11874122  Li, YF. et al. 2002. Anal Sci. 18: 177-181.

PMID: # 7687447  Chu, YH. et al. 1993. BioTechniques. 14: 925-930.

PMID: # 3697949  Giri, AK. et al. 1986. Cancer Lett. 30: 315-320.

PMID: # 6190068  Wilson, CM. et al. 1983. Meth. Enzymol. 91: 236-247.

PMID: # 6160787  Allen, RE. et al. 1980. Anal. Biochem. 104: 494-498.

Citations 1 to 6 of 6 total
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Rated 5 out of 5 by from Various publications cite the use of Fast Various publications cite the use of Fast Green FCF in IEF and SDS-PAGE tissue and protein staining. Has the ability to determine efficiency of transfer and localization of sample proteins on blotting membranes. Other applications of Fast Green FCF include using its clastogenic properties and it may also act at a presynaptic locus inhibiting neurotransmitter release in whole-cell voltage clamped hippocampal interneurons. -SCBT Publication Review
Date published: 2015-04-08
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