c-Jun Antibody (H-79) DISCONTINUED: recommended for detection of c-Jun p39 of mouse, rat, human and x origin by WB, IP, IF, IHC(P), FCM and ELISA

c-Jun Antibody (H-79): sc-1694

c-Jun Antibody (H-79) is rated 4.6 out of 5 by 11.
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    • c-Jun Antibody (H-79) is a rabbit polyclonal IgG; 200 µg/ml
    • epitope corresponding to amino acids 1-79 mapping at the N-terminus of c-Jun of human origin
    • recommended for detection of c-Jun p39 of mouse, rat, human and x origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and canine, bovine and porcine
Monos, satisfaction guaranteed

c-Jun (H-79) has been discontinued and replaced by c-Jun (G-4): sc-74543. This new monoclonal antibody is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

Ordering Information

c-Jun (G-4): sc-74543 [ Recommended monoclonal replacement for c-Jun (H-79) ]
PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
c-Jun Antibody (G-4) sc-74543 200 µg/ml $284.00
c-Jun Antibody (G-4) AC sc-74543 AC 500 µg/ml, 25% agarose $372.00
c-Jun Antibody (G-4) Alexa Fluor® 488 sc-74543 AF488 200 µg/ml $320.00
c-Jun Antibody (G-4) Alexa Fluor® 647 sc-74543 AF647 200 µg/ml $320.00
c-Jun Antibody (G-4) FITC sc-74543 FITC 200 µg/ml $297.00
c-Jun Antibody (G-4) HRP sc-74543 HRP 200 µg/ml $284.00
c-Jun Antibody (G-4) PE sc-74543 PE 200 µg/ml $308.00
c-Jun Antibody (G-4) X sc-74543 X 200 µg/0.1 ml $284.00

Quelle est la concentration recommandée pour une utilisation en FCM? Merci

Asked by: abbas89
Merci pour votre requête. En cytométrie en flux (FCM), nous suggérons d'utiliser cet anticorps à une concentration de 1 µg pour 1 x 10^6 cellules. Vous trouverez plus de précisions dans nos protocoles disponibles en ligne. Thank you very much for your inquiry. For FCM applications, we suggest this antibody in a concentration of 1 µg per 1 x 10^6 cells. You will also find some additional information in our protocols available online.
Answered by: Technical Support Europe
Date published: 2017-09-26
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Rated 4 out of 5 by from Used to detect c-Jun protein levels This antibody is very useful for cell compartment verification in animal tissue. In particular, we used this antibody to check the purity of nuclear cell extracts and to check any possible contamination by nuclear proteins in other cell compartments, such are cytosol or mytosol brain tissue of mice and rats. In general, we are satisfied with the obtained results, because Ab displayed high purity and clear bands in Western blot. Dilution up to 1:1000 for Western blot.
Date published: 2017-11-23
Rated 4 out of 5 by from Used to detect c-Jun levels This antibody is very useful for cell compartment verification in animal tissue. In particular, we used this antibody to check the purity of nuclear cell extracts and to check any possible contamination by nuclear proteins in other cell compartments, such are cytosol or mytosol brain tissue of mice and rats. In general, we are satisfied with the obtained results, because Ab displayed high purity and clear bands in Western blot. Dilution up to 1:1000 for Western blot.
Date published: 2017-11-23
Rated 5 out of 5 by from excellent excellent
Date published: 2015-11-19
Rated 3 out of 5 by from Decent anti Decent anti-c-Jun antibody. The signal is quite weak and sometimes dirty. This could, however, be because c-Jun is not so abundant in the cell.
Date published: 2015-11-11
Rated 5 out of 5 by from Recently published with success Recently published with success. WB, Human/Mouse: MEFs, HEK 293, HeLa, SiHa, C33A, HUVECs, recombinant protein.
Date published: 2015-10-27
Rated 5 out of 5 by from Used the AF647 in flow and works very well Used the AF647 in flow and works very well.
Date published: 2015-10-19
Rated 5 out of 5 by from Great specificity in WB in untreated and Great specificity in WB in untreated and phorbol ester-induced NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Produced excellent intracellular FCM data Produced excellent intracellular FCM data for fixed and permeabilized control and PMA induced NIH/3T3 cells. -SCBT QC
Date published: 2015-04-17
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