beta Tubulin Antibody (D-10) recommended for detection of β Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

β Tubulin Antibody (D-10): sc-5274

β Tubulin Antibody (D-10) is rated 4.7 out of 5 by 75.
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Datasheets
    • β Tubulin Antibody (D-10) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin
    • recommended for detection of β Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of β Tubulin (D-10): sc-5274.
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What is the lower band (~33kDa) in the western blot image shown on this webpage for sample (D)-KNRK whole cell lysate? I observed this band in my HeLa whole cell lysate as well.

Asked by: Kayla08
Thank you for your question. Unfortunately this is not a band that we consistently see in our in house testing, so we have not looked into this non-specific band. We do not see it in Hela cells, so I am not sure what you could be seeing. The antibody does not cross-react with anything that should run at that size. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-05-29

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-21

Which isoform of beta tubulin does this antibody recognize?

Asked by: eleni
Thank you for your question. Unfortunately the exact epitope of this antibody has not been mapped. It is raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin, accession#P07437. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-05-09
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Rated 5 out of 5 by from Tubulin antibody works great! We used this antibody to normalize the signals in Wb, it works great at 1:1000 dilution.
Date published: 2018-08-04
Rated 1 out of 5 by from any band on WB I received this antibody a couple of month ago and we tried three times in different cell lysates at the concentration of 1:100 and using as secondary the m-IgGκ BP-HRP at 1:1000 and we did not see any band.
Date published: 2018-04-23
Rated 5 out of 5 by from Good TLR4 antibody Sometimes non-specific bands occur during westerns, but I have not experienced this with the TLR4 antibody.
Date published: 2017-12-20
Rated 5 out of 5 by from good clear mark I tested in WB, 1:2000 for neuronal cell culture and worked very well
Date published: 2017-12-05
Rated 5 out of 5 by from Good Impression for Us The antibody showed strong and clear detection of ∫ Tubulin. The quality is good and there is no band, which is suitable for WB.
Date published: 2017-11-02
Rated 5 out of 5 by from Very Strong Signal We detected a strong band in the KNRK (D) whole cell line, clearly seen in 55kDa, the results were satisfactory.
Date published: 2017-10-27
Rated 5 out of 5 by from Clear and specific band I got this sample a month ago, and in western blot, it can get a clear and specific band.
Date published: 2017-10-26
Rated 5 out of 5 by from great I used this antibody for WB on human blood samples, it works very well.
Date published: 2017-10-23
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