Ammonium SulfateA reagent used in molecular biology and chromatography

Ammonium Sulfate (CAS 7783-20-2)

Ammonium Sulfate | CAS 7783-20-2 is rated 5.0 out of 5 by 1.
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Synonym: Mascagnite
Application: A reagent used in molecular biology and chromatography
CAS Number: 7783-20-2
Purity: ≥99%
Molecular Weight: 132.1
Molecular Formula: (NH4)2SO4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ammonium sulfate is a widely used reagent in molecular biology and chromatography. Common applications include precipitation and fractionation of proteins, crystallization of proteins and protein-nucleic acid complexes. Ammonium sulfate use has been reported in long PCR buffer, PCR lysis solution, second-strand cDNA synthesis, purification of antibodies, and to manufacture sulfuric acid.


References

1. Englard, S. and Seifter, S. 1990. Meth. Enzymol. 182: 285-300. PMID: 2314242
2. Green, A. A., and Hughes, W. L. 1955. Methods Enzymol. 1, 67-90
3. Kent, U.M. 1994. Methods Mol. Biol. 34: 13-21. PMID: 7711841
4. Kent, U.M. 1999. Methods Mol. Biol. 115: 11-18. PMID: 10098159
5. Mant, C. T., and Hodges, R. S. 1991. High- Performance Liquid Chromatography of Peptides and Proteins, Mant, C. T., and Hodges, R. S., eds. CRC Press. Boca Raton, FL:. pp. 437-450
6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor. Vol. 3.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (132 g/L) at 20 °C, and water (77 g/100 mL) at 25 °C. Insoluble in acetone, alcohol, and ether.
Storage :
Store at room temperature
Melting Point :
>280° C (dec.)
Density :
1.17 g/cm3
Refractive Index :
n20D 1.40
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
BS4500000
PubChem CID :
6097028
Merck Index :
14: 555
MDL Number :
MFCD00003391
EC Number :
231-984-1
SMILES :
[NH4+].[NH4+].[O-]S(=O)(=O)[O-]

Download SDS (MSDS)

Certificate of Analysis

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Ammonium Sulfate  Product Citations

See how others have used Ammonium Sulfate. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 10 total

PMID: # 19002655  Dey, S. et al. 2009. Mol. Cell. Biochem. 322: 137-142.

PMID: # 19351701  Lu, J. et al. 2009. FASEB J. 23: 2394-2402.

PMID: # 19805344  Sharma, H. et al. 2009. Proc. Natl. Acad. Sci. U.S.A. 106: 16604-16609.

PMID: # 19843847  Ciró, M. et al. 2009. Cancer Res. 69: 8491-8498.

PMID: # 17151020  Orrù, S. et al. 2007. Mol. Cell Proteomics. 6: 382-393.

PMID: # 17172420  Madhankumar, AB. et al. 2006. Mol. Cancer Ther. 5: 3162-3169.

PMID: # 15199141  Wang, X. et al. 2004. Mol. Cell. Biol. 24(13): 5850-62.

PMID: # 15199141  Wang, X. et al. 2004. Mol. Cell. Biol. 24(13): 5850-62.

PMID: # 12138178  ten Hoeve J. et al. 2002. Mol Cell Biol. 22(16): 5662-5668.

PMID: # 11148210  Gao, Y. et al. 2001. J. Biol. Chem. 276: 9838-9845.

Citations 1 to 10 of 10 total
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Rated 5 out of 5 by from Worked very well in our experiments Worked very well in our experiments.
Date published: 2015-05-01
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