ABCG2 Antibody (BXP-21): sc-58222

ABCG2 Antibody (BXP-21) is a mouse monoclonal IgG2a antibody that detects ABCG2 in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ABCG2 antibody (BXP-21) is available in a non-conjugated form, allowing for versatile applications in research. The ATP-binding cassette (ABC) transporter family, to which ABCG2 belongs, plays a crucial role in the transport of various molecules across cellular membranes, utilizing energy derived from ATP hydrolysis. ABCG2, also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1), is particularly significant due to involvement in conferring resistance to chemotherapeutic agents, including anthracyclines and topotecan. This resistance mechanism impacts chemotherapy efficacy in cancer treatment. ABCG2 is a key player in stem cell biology, with widespread expression in various stem cell populations and responsibility for the side population (SP) phenotype associated with stem cell properties. ABCG2 is abundantly expressed in placenta, liver, intestine, and stem cells, highlighting a protective role in detoxifying cells and regulating stem cell differentiation. ABCG2 may undergo post-translational modifications such as N-linked glycosylation and can dimerize in vivo, influencing function and protein interactions. ABCG2 (BXP-21) antibody serves as a valuable tool for researchers investigating roles in drug resistance and stem cell biology.

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ABCG2 Antibody (BXP-21) References:

1. Characterization of neurosphere cell phenotypes by flow cytometry.  |  Hulspas, R. and Quesenberry, PJ. 2000. Cytometry. 40: 245-50. PMID: 10878568
2. ABC transporters as phenotypic markers and functional regulators of stem cells.  |  Bunting, KD. 2002. Stem Cells. 20: 11-20. PMID: 11796918
3. Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells in vivo.  |  Zhou, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 12339-44. PMID: 12218177
4. Multidrug resistance and cancer: the role of the human ABC transporter ABCG2.  |  Ejendal, KF. and Hrycyna, CA. 2002. Curr Protein Pept Sci. 3: 503-11. PMID: 12369998
5. Characterization of drug transport, ATP hydrolysis, and nucleotide trapping by the human ABCG2 multidrug transporter. Modulation of substrate specificity by a point mutation.  |  Ozvegy, C., et al. 2002. J Biol Chem. 277: 47980-90. PMID: 12374800
6. ABCG2 confers resistance to indolocarbazole compounds by ATP-dependent transport.  |  Nakagawa, R., et al. 2002. Biochem Biophys Res Commun. 299: 669-75. PMID: 12459192
7. Characterization of a side population of cancer cells from human gastrointestinal system.  |  Haraguchi, N., et al. 2006. Stem Cells. 24: 506-13. PMID: 16239320
8. Resting and activated subsets of mouse multipotent hematopoietic stem cells.  |  Spangrude, GJ. and Johnson, GR. 1990. Proc Natl Acad Sci U S A. 87: 7433-7. PMID: 1977160
9. Tanshinone I, a new EZH2 inhibitor restricts normal and malignant hematopoiesis through upregulation of MMP9 and ABCG2.  |  Huang, Y., et al. 2021. Theranostics. 11: 6891-6904. PMID: 34093860
10. ABCG2 is an itaconate exporter that limits antibacterial innate immunity by alleviating TFEB-dependent lysosomal biogenesis.  |  Chen, C., et al. 2024. Cell Metab. 36: 498-510.e11. PMID: 38181789
11. ABCG2-Expressing Clonal Repopulating Endothelial Cells Serve to Form and Maintain Blood Vessels.  |  Lin, Y., et al. 2024. Circulation. 150: 451-465. PMID: 38682338
12. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species.  |  Goodell, MA., et al. 1997. Nat Med. 3: 1337-45. PMID: 9396603

The preferred ABCG2 antibody is ABCG2 Antibody (BXP-53): sc-58224.