Molecular structure of 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, CAS Number: 37067-30-4
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (CAS 37067-30-4)
Alternate Names:
4-Methylumbelliferyl 2-Acetamido-2-deoxy-β-D-glucopyranoside
CAS Number:
37067-30-4
Molecular Weight:
379.40
Molecular Formula:
C18H21NO8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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4-Methylumbelliferyl N-acetyl-β-D-glucosaminide is a substrate enzymatic assays to measure the activity of enzymes such as chitinases and chitobiases. When acted upon by these enzymes, the substrate is cleaved, releasing the fluorescent compound 4-methylumbelliferone. This fluorescence can be quantified using a fluorometer, allowing for the measurement of enzyme activity. The mechanism of action involves the enzymatic hydrolysis of the glycosidic bond between the N-acetylglucosamine and the 4-methylumbelliferone moiety, resulting in the release of the fluorescent product. This allows for the precise measurement of enzyme activity in a variety of experimental applications, providing insights into the function and regulation of these enzymes.
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (CAS 37067-30-4) References
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- Characterization of beta-hexosaminidase secretion in rabbit lacrimal gland. | Andersson, SV., et al. 2006. Exp Eye Res. 83: 1081-8. PMID: 16839547
- Structural and kinetic analysis of Bacillus subtilis N-acetylglucosaminidase reveals a unique Asp-His dyad mechanism. | Litzinger, S., et al. 2010. J Biol Chem. 285: 35675-84. PMID: 20826810
- Cloning and characterization of a bifunctional glycosyl hydrolase from an antagonistic Pseudomonas putida strain P3(4). | Singh, NA. and Shanmugam, V. 2012. J Basic Microbiol. 52: 340-9. PMID: 21953214
- Thiamet-G-mediated inhibition of O-GlcNAcase sensitizes human leukemia cells to microtubule-stabilizing agent paclitaxel. | Ding, N., et al. 2014. Biochem Biophys Res Commun. 453: 392-7. PMID: 25268318
- N-acetyl-beta-D-glucosaminidase assay in urine: urea inhibition. | Mueller, PW., et al. 1989. J Anal Toxicol. 13: 188-90. PMID: 2666744
- Porous Silicon Fabry-Pérot Interferometer for N-Acetyl-β-d-Glucosaminidase Biomarker Monitoring. | Kumar, DN., et al. 2020. ACS Sens. 5: 1969-1976. PMID: 32573203
- A rapid test for chitinase activity that uses 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide. | O'Brien, M. and Colwell, RR. 1987. Appl Environ Microbiol. 53: 1718-20. PMID: 3662513
- Isolation of Momordica charantia seed lectin and glycosidases from the protein bodies: Lectin-glycosidase (β-hexosaminidase) protein body membrane interaction reveals possible physiological function of the lectin. | Vutharadhi, S. and Nadimpalli, SK. 2023. Plant Physiol Biochem. 197: 107663. PMID: 36989986
- Cloning, expression, purification and biochemical characterization of the recombinant chitinase enzyme encoded by CTS2 in the budding yeast. | Jiang, L. and Yan, H. 2023. Protein Expr Purif. 208-209: 106294. PMID: 37150231
- A microtiter plate assay for N-acetyl-beta-D-glucosaminidase using a fluorogenic substrate. | Linko-Löppönen, S. and Mäkinen, M. 1985. Anal Biochem. 148: 50-3. PMID: 4037307
- N-Acetyl-beta-glucosaminidases in human spleen. | Robinson, D. and Stirling, JL. 1968. Biochem J. 107: 321-7. PMID: 5650361
- Purification and characterization of N-acetylglucosaminidase from Trypanosoma cruzi. | el Moudni, B., et al. 1996. Exp Parasitol. 83: 167-73. PMID: 8682185
- Urine N-acetyl-beta-D-glucosaminidase activity in healthy cattle. | Sato, R., et al. 1997. Am J Vet Res. 58: 1197-200. PMID: 9361877
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Enzyme.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 250 mg | sc-252184 | 250 mg | $120.00 | |||
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 500 mg | sc-252184A | 500 mg | $150.00 | |||
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 1 g | sc-252184B | 1 g | $200.00 | |||
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 2 g | sc-252184C | 2 g | $330.00 | |||
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 5 g | sc-252184D | 5 g | $765.00 | |||
4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, 10 g | sc-252184E | 10 g | $1370.00 |