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3,3′-Diaminobenzidine (CAS 91-95-2)

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Alternate Names:
3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl; DAB
Application:
3,3′-Diaminobenzidine is A peroxidase substrate and a reagent for spectrophotometric determination of selenium
CAS Number:
91-95-2
Purity:
≥98%
Molecular Weight:
214.27
Molecular Formula:
C12H14N4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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3,3′-Diaminobenzidine (DAB) finds extensive utility across diverse scientific research applications. Its versatility allows for the synthesis of various compounds, facilitating a broad spectrum of biochemical and physiological effects. In laboratory experiments, 3,3′-Diaminobenzidine has been studied extensively for potential medical applications. The applications of 3,3′-Diaminobenzidine in scientific research are multifaceted. It serves as a component in dye synthesis, pharmaceutical development, and investigations pertaining to enzyme kinetics. Moreover, its utilization spans protein-protein interaction studies, gene expression analysis, and investigations into cell signaling pathways. 3,3′-Diaminobenzidine has also been instrumental in elucidating cell metabolism, cell adhesion phenomena, and cell cycle regulation. Interacting with an array of molecules, such as proteins, enzymes, and DNA, 3,3′-Diaminobenzidine showcases its adaptability. It forms covalent bonds with amino acid residues of proteins, facilitating interactions. Furthermore, it engages with DNA by establishing covalent bonds with nucleic acid bases. Enzymes also experience the influence of 3,3′-Diaminobenzidine, with covalent bonding occurring at the active sites of these catalysts. The remarkable properties of 3,3′-Diaminobenzidine, along with its ability to interact with diverse molecular entities, have made it an invaluable tool in scientific research. Its multifunctionality continues to unlock new avenues for exploration in various fields.


3,3′-Diaminobenzidine (CAS 91-95-2) References

  1. Facile isolation of bisimines based on 3,3'-diaminobenzidine: direct access to unsymmetrical bimetallic salphen building blocks.  |  Curreli, S., et al. 2007. J Org Chem. 72: 7018-21. PMID: 17676804
  2. Synthesis, spectroscopic studies and crystal structure of the Schiff base ligand L derived from condensation of 2-thiophenecarboxaldehyde and 3,3'-diaminobenzidine and its complexes with Co(II), Ni(II), Cu(II), Cd(II) and Hg(II): Comparative DNA binding studies of L and its Co(II), Ni(II) and Cu(II) complexes.  |  Shakir, M., et al. 2011. Spectrochim Acta A Mol Biomol Spectrosc. 79: 1866-75. PMID: 21715221
  3. Comparison of particulate 3,3',5,5'-tetramethylbenzidine and 3,3'-diaminobenzidine as chromogenic substrates for immunoblot.  |  Brand, JA., et al. 1990. Biotechniques. 8: 58-60. PMID: 2322454
  4. Molecular structure, spectroscopic characterization, HOMO and LUMO analysis of 3,3'-diaminobenzidine with DFT quantum chemical calculations.  |  Karabacak, M., et al. 2015. Spectrochim Acta A Mol Biomol Spectrosc. 150: 83-93. PMID: 26026306
  5. 3,3'-Diaminobenzidine staining interferes with PCR-based DNA analysis.  |  Dölle, C., et al. 2018. Sci Rep. 8: 1272. PMID: 29352159
  6. Contrary logic pairs and circuits using a visually and colorimetrically detectable redox system consisting of MoO3-x nanodots and 3,3'-diaminobenzidine.  |  Huang, W., et al. 2019. Mikrochim Acta. 186: 79. PMID: 30627952
  7. A field-applicable colorimetric assay for notorious explosive triacetone triperoxide through nanozyme-catalyzed irreversible oxidation of 3, 3'-diaminobenzidine.  |  Hormozi Jangi, SR., et al. 2020. Mikrochim Acta. 187: 431. PMID: 32632565
  8. DAB-quant: An open-source digital system for quantifying immunohistochemical staining with 3,3'-diaminobenzidine (DAB).  |  Patel, S., et al. 2022. PLoS One. 17: e0271593. PMID: 35857792
  9. Some important principles in 3,3'-diaminobenzidine ultrastructural cytochemistry.  |  Seligman, AM., et al. 1973. J Histochem Cytochem. 21: 756-8. PMID: 4125274
  10. A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.  |  Herzog, V. and Fahimi, HD. 1973. Anal Biochem. 55: 554-62. PMID: 4750690
  11. Immunoperoxidase methods: increased efficiency using fluorescence microscopy for 3,3-diaminobenzidine (DAB) stained semithin sections.  |  Grube, D. 1980. Histochemistry. 70: 19-22. PMID: 6161911
  12. Selective cytochemical localization of peroxidase, cytochrome oxidase and catalase in rat liver with 3,3'-diaminobenzidine.  |  Angermüller, S. and Fahimi, HD. 1981. Histochemistry. 71: 33-44. PMID: 6262282
  13. Transition metal-catalysed oxidation of 3,3'-diaminobenzidine [DAB] in a model system.  |  Litwin, JA. 1982. Acta Histochem. 71: 111-7. PMID: 6815957

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3,3′-Diaminobenzidine, 1 g

sc-216567
1 g
$32.00

3,3′-Diaminobenzidine, 5 g

sc-216567A
5 g
$64.00

3,3′-Diaminobenzidine, 25 g

sc-216567B
25 g
$183.00

3,3′-Diaminobenzidine, 100 g

sc-216567C
100 g
$590.00

3,3′-Diaminobenzidine, 250 g

sc-216567D
250 g
$1358.00

bonsoir je voudrais savoir comment rengréner le diaminobenzidine (DAB) en poudre ???

Asked by: nesrine
Merci pour votre question. Veuillez contacter notre Service Technique pour toute information complémentaire concernant ce produit. Thank you for your inquiry. Please contact our Technical Service Department for additional information regarding this product.
Answered by: Technical Support Europe
Date published: 2022-09-02
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Rated 4 out of 5 by from Definitely IS for IHCI just bought and stained using this, and it works well. Just like any other DAB that I've used before. Just mixed it in dH20, so it will be in suspension and then aliquotted it as per the normal IHC protocol. (I don't think it will ever dissolve, but it does develop into a brown stain). I would recommend pouring the powder into a bigger container first and then mixing it for a long time in water at the concentration you need.
Date published: 2019-08-20
Rated 1 out of 5 by from NOT for IHCI bought this product by accident, thinking it was the diaminobenzidine that is used in IHC (immunohistochemistry) as a substrate for horse radish peroxidase, but that is NOT what this is. It is NOT water soluble at all and is a BLACK flaky powder. And any residual solubility it might have does NOT develop the brown DAB reaction of peroxidase. I couldn't dissolve it in acid, base, ethanol, or DMSO either.
Date published: 2018-04-11
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3,3′-Diaminobenzidine is rated 2.5 out of 5 by 2.
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