Date published: 2025-10-2

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m-IgGκ BP-HRP: sc-516102

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Datasheets
  • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP)
  • supplied at 200 µg in 0.5 ml volume
  • Highly recommended alternative to conventional anti-mouse IgG secondary antibodies for chemiluminescence Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
  • Suitable for binding to most, but not all, mouse IgGκ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGλ light chain antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • For a Cruz Marker™ compatible mouse IgG kappa binding protein, use m-IgGκ BP-HRP (Cruz Marker) (sc-516102-CM) (Click here for datasheet)
  • For mouse IgGλ immunoglobulins we recommend m-IgGλ BP-HRP (sc-516132)
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
  • Also see our new m-IgGκ BP-FITC, PE, CFL 488, CFL 555, CFL 594, CFL 647, CFL 680 and CFL 790 fluorescent dye conjugates. These represent substantial improvements compared to conventional polyclonal anti-mouse secondary antibody fluorescent dye conjugates.

QUICK LINKS

Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to horseradish peroxidase (HRP) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB) and immunohistochemical (IHC) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies.

For a Cruz Marker™ compatible mouse IgG kappa binding protein, use m-IgGκ BP-HRP (Cruz Marker) (sc-516102-CM).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

m-IgGκ BP-HRP

sc-516102
200 µg/0.5 ml
$92.00

m-IgGκ BP-HRP (Cruz Marker)

sc-516102-CM
200 µg/0.5 ml
$92.00

I recently purchased sc-516102 m-IgGκ BP-HRP since sc-2005 goat anti-mouse IgG-HRP is no longer available. I compared them in WB against SC-32233 GAPDH and B-sc-47778 β-Actin. However, the detection result with the new sc-516102 is worse compared to sc-20

Asked by: mignogna1963
We are sorry to hear that this antibody did not perform as expected. We want you to be successful in your research, so please contact our Technical Service Department by phone, e-mail or live chat.
Answered by: BlakeJ
Date published: 2025-09-12

含まれる毒物の名前は?

Asked by: ホリウチ
Thank you for your question.The antibody components are: Each vial contains 200 µg mouse IgGκ binding protein-HRP in 0.5 ml of PBS containing 40% glycerol, 1% stabilizer protein and < 0.01% thimerosal.No other components are present.Thank you. ご質問ありがとうございます。抗体成分は以下の通りです:各バイアルには、0.5 mlのPBS(40%グリセロール、1%安定化タンパク質、および<0.01%チメロサールを含む)中に、マウスIgGκ結合タンパク質-HRPが200 µg含まれています。その他の成分は含まれていません。
Answered by: Jiajun Z
Date published: 2025-08-25

For western blots, will the standard H+L secondary HRP not work? Does it have to be IgG1 kappa light chain- HRP secondary?

Asked by: Everardo
Thank you for your question. For assistance please contact Technical Support. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-23

Can this antibody be used as a secondary antibody for sc-515355?

Asked by: 1234
Thank you for your question. At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIBCH Antibody (E-11).
Answered by: BlakeJ
Date published: 2025-03-27

Is this a biotinylated secondary antibody? will it need to be conjugated to biotin separately if used in IHC protocol? Thinking of getting this as a secondary antibody to your (B-3): sc-8391 primary antibody.

Asked by: Alana
Thank you for your question. sc-516102 m-IgGκ BP-HRP is not a biotinylated secondary antibody . Here is a recommended biotin secondary for IHC. https://www.scbt.com/p/m-igg1-bp-b
Answered by: BlakeJ
Date published: 2025-01-30

which myc antibody is conjugated with HRP?

Asked by: qingtian
Thank you for your question. Here is a popular Myc antibody : https://www.scbt.com/p/c-myc-antibody-9e10
Answered by: BlakeJ
Date published: 2024-08-09

Good morning. What reagent should this product be diluted for use in Western blotting? I am thinking of 【UltraCruz® Blocking Reagent: sc-516214】. We are sorry for your busy schedule, but we would appreciate your answer.

Asked by: ogacho
Thank you for your question. You can use PBS, PBST, TBS, TBST. all four of these can be mixed with the appropriate of skim milk powder (1%-5% non-fat milk), or (1%-5% BSA). Thank you.
Answered by: Jiajun Z
Date published: 2024-07-03

Will this reagent also detect a mouse IgM monoclonal with kappa light chains?

Asked by: Benk
Thank you for your question. Yes, kappa light chains in mouse IgM and IgA primary antibodies will also be detected.
Answered by: Tech Support Europe
Date published: 2023-10-10
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Rated 5 out of 5 by from Excelente resultadoEs el anticuerpo secundario que utilizo de forma constante.
Date published: 2025-07-02
Rated 5 out of 5 by from Great secondary antibodyGreat Secondary Antibody (m-IgG BP-HRP sc-516102; LOT# J2924). The shipping was really fast. The quality is good at a reasonable price.
Date published: 2025-05-06
Rated 5 out of 5 by from Detection of mouse proteins via chemiluminiscenceThis antibody played a crucial role in our investigations in western blot analysis. Providing a robust signal in chemiluminescent experiments, the Mouse HRP antibody demonstrated reliability and stability.
Date published: 2024-01-04
Rated 5 out of 5 by from Great for chemiluminescent western blots!This detection reagent worked well for detecting G9a (C-9) antibody, sc-515726, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Great for chemiluminescent western blots!This detection reagent worked well for detecting CRF (2B11) antibody, sc-293187, in a chemiluminescent western blot assay. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Secondary AntibodySecondary antibody 1/2000 dilution, works well in western blotting.
Date published: 2023-06-30
Rated 5 out of 5 by from 抗体很好用朋友推荐的,之前用其他的二抗效果不好,换了这个之后,跑出来了,杂带很少,抗体很好用,跑了几次,效果不错,背景很干净
Date published: 2023-06-14
Rated 5 out of 5 by from Working well!It worked beautifully as it is supposed to be with all of primary Abs we tried.
Date published: 2023-02-09
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m-IgGκ BP-HRP is rated 4.7 out of 5 by 338.
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