Oligonucléotides
Electrophoretic mobility shift assay (EMSA) or gel shift assay is a commonly used technique for understanding cellular and molecular mechanisms by analyzing protein-nucleic acid interactions. Santa Cruz Biotechnology offers an array of specialty reagents for gel shift assays including a selection of consensus and mutant Oligonucleotides. Oligonucleotides are composed of well characterized consensus DNA sequences for specific transcription factors of interest. Mutant oligonucleotides are also available with one or more site-directed mutations for competitive gel shift assays.
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| Empty Table Header | Nom du produit | CONSENSUS CAT. # | MUTANT CAT. # | MUTANT CONSENSUS | MOTIF DE LIAISON | CITATIONS | Classement |
|---|---|---|---|---|---|---|---|
Oligonucléotides AP-1 pour le Retard sur Gel | sc-2501 | sc-2514 | 5′-CGC TTG ATG ACT CAG CCG GAA-3′ | "CA"→"TG" | 542 | ||
Sp1 consensus oligonucleotide | sc-2502 | sc-2503 | 5′-ATT CGA TCG GGG CGG GGC GAG C-3′ | "GG"→"TT" | 258 | ||
CREB consensus oligonucleotide | sc-2504 | sc-2517 | 5’-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3’ | "AC"→"TG" | 116 | ||
NFκB consensus oligonucleotide | sc-2505 | sc-2511 | 5'-AGT TGA GGG GAC TTT CCC AGG C-3' | "G"→"C" | 1,104 | ||
Oct-1 consensus oligonucleotide | sc-2506 | sc-2515 | 5’-TGT CGA ATG CAA ATC ACT AGA A-3’ | "AT"→"GC" | 75 | ||
E2F-1 consensus oligonucleotide | sc-2507 | sc-2508 | 5-’ATT TAA GTT TCG CGC CCT TTC TCA A-3’ | "CG"→"AT" | 47 | ||
Myc-Max consensus oligonucleotide | sc-2509 | sc-2510 | 5’-GGA AGC AGA CCA CGT GGT CTG CTT CC-3’ | "TG"→"GA" | 37 | ||
AP-2α consensus oligonucleotide | sc-2513 | sc-2516 | 5'-GAT CGA ACT GAC CGC CCG CGG CCC GT-3' | "CC"→"TT" | 50 | ||
SRE consensus oligonucleotide | sc-2523 | sc-2524 | 5′-GGA TGT CCA TAT TAG GAC ATC T-3′ | "GG"→"TT" | 21 | ||
C/EBP consensus oligonucleotide | sc-2525 | sc-2526 | 5′-TGC AGA TTG CGC AAT CTG CA-3′ | "TTG CGC AA"→"GAC TAG TC" | 147 | ||
NF-E2 consensus oligonucleotide | sc-2527 | sc-2528 | 5’-TGG GGA ACC TGT GCT GAG TCA CTG GAG-3’ | "GA"→"AG" | 10 | ||
GATA consensus oligonucleotide | sc-2531 | sc-2532 | 5’-CAC TTG ATA ACA GAA AGT GAT AAC TCT-3’ | "GA"→"CT" | 54 | ||
YY1 consensus oligonucleotide | sc-2533 | sc-2534 | 5'-CGC TCC CCG GCC ATC TTG GCG GCT GGT-3' | "C"→"G" and "GCC"→ "ATT" | 27 | ||
SIE consensus oligonucleotide | sc-2535 | sc-2536 | 5’-GTG CAT TTC CCG TAA ATC TTG TCT ACA-3’ | "TTC"→"CCA" | 38 | ||
GAS/ISRE consensus oligonucleotide | sc-2537 | sc-2538 | 5'-AAG TAC TTT CAG TTT CAT ATT ACT CTA-3' | "TTCA"→"GGTC" | 34 | ||
GR consensus oligonucleotide | sc-2545 | sc-2546 | 5’-AGA GGA TCT GTA CAG GAT GTT CTA GAT-3’ | "GT"→"CA" | 22 | ||
RXR (DR-1) consensus oligonucleotide | sc-2547 | sc-2548 | 5’-AGC TTC AGG TCA GAG GTC AGA GAG CT-3’ | "GT"→"CA" | 8 | ||
AR consensus oligonucleotide | sc-2551 | sc-2552 | 5′-GAA GTC TGG TAC AGG GTG TTC TTT TTG-3′ | "GT"→"CA" | 13 | ||
NF-1 consensus oligonucleotide | sc-2553 | sc-2554 | 5’-TTT TGG ATT GAA GCC AAT ATG ATA A-3’ | "GCC"→"TAA" | 32 | ||
RAR (DR-5) consensus oligonucleotide | sc-2559 | sc-2560 | 5’-AGG GTA GGG TTC ACC GAA AGT TCA CTC-3’ | "TT"→"AA" | 10 | ||
Stat5 consensus oligonucleotide | sc-2565 | sc-2566 | 5’-AGA TTT CTA GGA ATT CAA TCC-3’ | "CTAGG"→"AGTTT" | 49 | ||
Stat5/Stat6 consensus oligonucleotide | sc-2567 | sc-2568 | 5’-GTA TTT CCC AGA AAA GGA AC-3’ | "CCAG"→"GGTT" | 20 | ||
Stat3 consensus oligonucleotide | sc-2571 | sc-2572 | 5’-GAT CCT TCT GGG AAT TCC TAG ATC-3’ | "AAT"→"CCG" | 145 | ||
Stat1 consensus oligonucleotide | sc-2573 | sc-2574 | 5’-CAT GTT ATG CAT ATT CCT GTA AGT G-3’ | "CCT"→"GGA" | 66 | ||
IRF-1 consensus oligonucleotide | sc-2575 | sc-2576 | 5’-GGA AGC GAA AAT GAA ATT GAC T-3’ | "AA"→"GG" | 25 | ||
NFATc consensus oligonucleotide | sc-2577 | sc-2578 | 5’-CGC CCA AAG AGG AAA ATT TGT TTC ATA-3’ | "AGG"→"CTT" | 49 | ||
p53 consensus oligonucleotide | sc-2579 | sc-2580 | 5'-TAC AGA ACA TGT CTA AGC ATG CTG GGG-3' | "CATG"→"TCGC" | 59 | ||
c-Myb consensus oligonucleotide | sc-2583 | sc-2584 | 5’-TAC AGG CAT AAC GGT TCC GTA GTG A-3’ | "A"→"T" | 8 | ||
ER consensus oligonucleotide | sc-2585 | sc-2586 | 5′-GGA TCT AGG TCA CTG TGA CCC CGG ATC-3′ | "GT"→"TA" | 15 | ||
PPAR consensus oligonucleotide | sc-2587 | sc-2588 | 5'-AG GTC AAA GGT CA-3' | "GT"→"CA" | 40 | ||
Brn-3 consensus oligonucleotide | sc-2595 | sc-2596 | 5’-CAC AGC TCA TTA ACG CGC-3’ | "TT"→"GC" | 1 | ||
Smad3/4 consensus oligonucleotide | sc-2597 | sc-2598 | 5’-TCG AGA GCC AGA CAA AAA GCC AGA CAT TTA GCC AGA CAC-3’ | "C"→"T" and "G"→"C" | 16 | ||
Smad SBE consensus oligonucleotide | sc-2603 | sc-2604 | 5'-G TCT AGA C CA-3' | "GTCTAGAC"→"CATAGCGT" | 7 | ||
CTCF consensus oligonucleotide | sc-2613 | sc-2614 | 5′-GGC GGC GCC GCT AGG GGT CTC TCT-3′ | "GCCGCT"→"TAATAG" | 1 | ||
TFEB consensus oligonucleotide | sc-2621 | sc-2622 | 5'-C ACG TG-3' | "G"→"C" | 1 | ||
Gfi-1 consensus oligonucleotide | sc-2623 | sc-2624 | 5'-T AA ATC ACT GC-3' | "A"→"C" | 0 | ||
HIF-1α consensus oligonucleotide | sc-2625 | sc-2626 | 5'-TCT GTA CGT GAC CAC ACT CAC CTC-3' | "CGT"→"AAA" | 27 |