ZNF434 Double Nickase Plasmid (h): sc-412383-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF434 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF434 Double Nickase Plasmid (h) and ZNF434 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZSCAN32. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF434 Double Nickase Plasmid (h) | sc-412383-NIC | 20 µg | $410.00 | |||
ZNF434 Double Nickase Plasmid (h2) | sc-412383-NIC-2 | 20 µg | $410.00 |
ZSCAN32 encodes a zinc finger protein (ZNF434) that is predicted to function as a sequence-specific DNA-binding transcriptional regulator, consistent with the C2H2-type zinc finger family’s role in modulating chromatin state and gene expression programs. By influencing transcriptional networks, ZNF434 is relevant to cellular processes such as differentiation, proliferation control, and stress-responsive signaling, where tight regulation of target promoters and enhancers is required. Dysregulated transcription factor activity and altered chromatin regulation are common features of tumorigenesis and immune-related dysfunction, making ZSCAN32/ZNF434 a useful locus for mechanistic studies in disease-relevant models. Investigating ZNF434 can help define downstream gene circuits and pathway perturbations associated with aberrant transcriptional regulation.
ZNF434 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZSCAN32 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZSCAN32. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZSCAN32 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZSCAN32-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.