ZIP10 CRISPR/Cas9 KO Plasmid (h): sc-406811
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZIP10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZIP10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZIP10 Antibody (1F6): sc-517167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZIP10 CRISPR/Cas9 KO Plasmid (h) | sc-406811 | 20 µg | $397.00 |
Overview
SLC39A10 encodes ZIP10, a plasma membrane zinc influx transporter in the SLC39/ZIP family that regulates cytosolic Zn2+ availability for metalloenzyme activity, zinc-finger transcription factors, and redox-sensitive signaling. By controlling zinc homeostasis, ZIP10 influences pathways linked to cell proliferation, differentiation, and stress responses, including signaling cascades shaped by zinc-dependent modulation of kinase and phosphatase activities. Altered ZIP10 expression or zinc handling has been associated in the literature with immune cell function, epithelial biology, and tumor-related phenotypes, reflecting the broad dependence of cellular programs on zinc availability. These features make SLC39A10 a relevant target for mechanistic studies of zinc-regulated signaling networks and metal ion transport biology.
ZIP10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC39A10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC39A10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC39A10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZIP10 protein expression.
This CRISPR knockout system enables efficient generation of SLC39A10-deficient cell models for investigation of ZIP10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC39A10 exon(s) critical for ZIP10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC39A10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZIP10 CRISPR/Cas9 KO Plasmid (h) and ZIP10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC39A10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZIP10 HDR Plasmid (h) and ZIP10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC39A10 homology arms to support homology-directed repair at defined SLC39A10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.