ZEB1 Double Nickase Plasmid (m): sc-423302-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZEB1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZEB1 Double Nickase Plasmid (m) and ZEB1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zeb1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZEB1 Double Nickase Plasmid (m) | sc-423302-NIC | 20 µg | $410.00 | |||
ZEB1 Double Nickase Plasmid (m2) | sc-423302-NIC-2 | 20 µg | $410.00 |
Zeb1 encodes the zinc finger E-box binding homeobox 1 (ZEB1), a sequence-specific transcription factor that binds E-box motifs to coordinate epithelial–mesenchymal transition (EMT), cell polarity, and lineage plasticity. In mouse systems, ZEB1 integrates TGF-β/SMAD signaling and cross-regulates microRNA networks such as the miR-200 family, reshaping transcriptional programs that control adhesion, migration, and differentiation. ZEB1 modulates chromatin and transcription through interactions with corepressors including CtBP and other epigenetic regulators, linking developmental cues to stable gene expression states. Dysregulated ZEB1 activity is associated with aberrant EMT-like programs and altered differentiation in disease-relevant models, making it a common target for mechanistic studies of invasion, fibrosis, and tumor progression pathways.
ZEB1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zeb1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zeb1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zeb1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zeb1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.