ZAP-70 Antibody (4H386): sc-73279

ZAP-70 Antibody (4H386) is a mouse monoclonal IgG1 antibody that detects ZAP-70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm, ZAP-70 associates with the ζ chain of the T cell receptor complex, where ZAP-70 becomes activated through phosphorylation in response to antigen engagement. This activation initiates a cascade of downstream signaling events that lead to T cell proliferation, differentiation, and cytokine production, ultimately shaping the adaptive immune response. The importance of ZAP-70 is underscored by ZAP-70′s involvement in various immunological disorders; mutations in the ZAP-70 gene can lead to severe combined immunodeficiency, highlighting ZAP-70′s critical function in maintaining effective immune system operations. Anti-ZAP-70 antibody (4H386) is an invaluable tool for researchers studying T cell signaling pathways and their implications in health and disease.

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ZAP-70 Antibody (4H386) References:

1. T cell development and T cell responses in mice with mutations affecting tyrosines 292 or 315 of the ZAP-70 protein tyrosine kinase.  |  Magnan, A., et al. 2001. J Exp Med. 194: 491-505. PMID: 11514605
2. Identification of a novel isoform of ZAP-70, truncated ZAP kinase.  |  Kuroyama, H., et al. 2004. Biochem Biophys Res Commun. 315: 935-41. PMID: 14985102
3. The B-cell receptor and ZAP-70 in chronic lymphocytic leukemia.  |  Kipps, TJ. 2007. Best Pract Res Clin Haematol. 20: 415-24. PMID: 17707830
4. Correlation between ZAP-70, phospho-ZAP-70, and phospho-Syk expression in leukemic cells from patients with CLL.  |  Kaplan, D., et al. 2010. Cytometry B Clin Cytom. 78: 115-22. PMID: 20014315
5. Peripheral blood lymphocyte phenotype of ZAP-70⁺ and ZAP-70⁻ patients with B-cell chronic lymphocytic leukaemia.  |  Rivkina, A., et al. 2015. Exp Oncol. 37: 73-6. PMID: 25804237
6. The Structural Basis for Activation and Inhibition of ZAP-70 Kinase Domain.  |  Huber, RG., et al. 2015. PLoS Comput Biol. 11: e1004560. PMID: 26473606
7. Negative regulation of TCR signaling by ubiquitination of Zap-70 Lys-217.  |  Ivanova, E. and Carpino, N. 2016. Mol Immunol. 73: 19-28. PMID: 27032069
8. T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.  |  Tewari, R., et al. 2021. J Biol Chem. 296: 100311. PMID: 33482200
9. Natural isoaspartyl protein modification of ZAP70 alters T cell responses in lupus.  |  Yang, ML., et al. 2023. Autoimmunity. 56: 2282945. PMID: 37994408
10. Interdomain B in ZAP-70 regulates but is not required for ZAP-70 signaling function in lymphocytes.  |  Zhao, Q., et al. 1999. Mol Cell Biol. 19: 948-56. PMID: 9858619

The preferred ZAP-70 antibody is ZAP-70 Antibody (1E7.2): sc-32760.