Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Yes Double Nickase Plasmid (h): sc-400261-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Yes Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Yes Double Nickase Plasmid (h) and Yes Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting YES1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Yes Antibody (C-10): sc-46674
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Yes Double Nickase Plasmid (h) | sc-400261-NIC | 20 µg | $410.00 | |||
Yes Double Nickase Plasmid (h2) | sc-400261-NIC-2 | 20 µg | $410.00 |
YES1 encodes Yes, a Src family non-receptor tyrosine kinase that relays signals from receptor tyrosine kinases, integrins, and immune receptors to downstream effectors controlling proliferation, adhesion, cytoskeletal dynamics, and survival. Yes participates in pathways such as focal adhesion signaling, MAPK/ERK, PI3K–AKT, and STAT regulation, linking extracellular cues to transcriptional and metabolic programs. Aberrant YES1 activity or copy-number gain has been associated with altered oncogenic signaling networks, including changes in cell migration and resistance-related kinase rewiring in multiple tumor contexts. As a signaling hub, YES1 is frequently studied for its role in phosphorylation-dependent pathway crosstalk and regulation of membrane-proximal signal transduction.
Yes Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the YES1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within YES1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt YES1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of YES1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.