Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XRN1 Double Nickase Plasmid (h): sc-401910-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XRN1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XRN1 Double Nickase Plasmid (h) and XRN1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XRN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XRN1 Antibody (C-1): sc-165985
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XRN1 Double Nickase Plasmid (h) | sc-401910-NIC | 20 µg | $410.00 | |||
XRN1 Double Nickase Plasmid (h2) | sc-401910-NIC-2 | 20 µg | $410.00 |
XRN1 encodes a conserved 5′→3′ exoribonuclease that drives cytoplasmic mRNA decay by degrading uncapped RNA after decapping, thereby shaping transcript turnover and RNA quality control. It functions at the intersection of decapping complexes, P-bodies, and co-translational surveillance pathways such as nonsense-mediated decay, helping coordinate gene expression programs during stress responses. By regulating the stability of specific transcripts, XRN1 influences innate immune signaling and broader RNA metabolism networks that are frequently perturbed in cancer and neurodevelopmental contexts. Dysregulated XRN1 activity has also been linked to altered antiviral responses and sensitivity to RNA virus replication, making it a useful node for studying host–pathogen interactions.
XRN1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XRN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XRN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XRN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XRN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.