Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XIAP Double Nickase Plasmid (h): sc-416497-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XIAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XIAP Double Nickase Plasmid (h) and XIAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XIAP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XIAP Antibody (A-7): sc-55550
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XIAP Double Nickase Plasmid (h) | sc-416497-NIC | 20 µg | $410.00 | |||
XIAP Double Nickase Plasmid (h2) | sc-416497-NIC-2 | 20 µg | $410.00 |
XIAP (X-linked inhibitor of apoptosis protein, BIRC4) is a cytosolic IAP family member that directly binds and suppresses caspase-3, -7, and -9, thereby modulating intrinsic and extrinsic apoptosis. Through its E3 ubiquitin ligase activity and interactions with RIPK2, TAK1, and TAB proteins, XIAP influences ubiquitin-dependent signaling that connects to NF-κB activation and inflammatory responses. XIAP also participates in crosstalk between cell death regulation and innate immunity, affecting thresholds for apoptosis in response to stress and cytokine cues. Dysregulated XIAP function has been associated with altered apoptosis sensitivity and immune signaling phenotypes, supporting its relevance in studies of cancer biology and immune-related pathophysiology.
XIAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XIAP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XIAP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XIAP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XIAP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.