X11α Double Nickase Plasmid (h): sc-405297-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- X11α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- X11α Double Nickase Plasmid (h) and X11α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APBA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: X11α Antibody (A-12): sc-137022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
X11α Double Nickase Plasmid (h) | sc-405297-NIC | 20 µg | $410.00 | |||
X11α Double Nickase Plasmid (h2) | sc-405297-NIC-2 | 20 µg | $410.00 |
APBA1 (X11α) is a neuronal adaptor protein that binds the amyloid precursor protein (APP) and coordinates protein trafficking, sorting, and synaptic signaling. Through its PTB and PDZ domains, X11α scaffolds complexes that influence vesicle exocytosis/endocytosis and postsynaptic organization, integrating processes relevant to neurotransmission and neuronal homeostasis. APBA1 has been linked to regulation of APP processing and amyloidogenic pathways, making it a widely used target for mechanistic studies in neurodegeneration and synaptic dysfunction. Altered APBA1-associated interactions have also been explored in the context of cognitive phenotypes and neuronal vulnerability, supporting its relevance in biomedical research models.
X11α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APBA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APBA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APBA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APBA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.