WDR82 Double Nickase Plasmid (h): sc-406963-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR82 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WDR82 Double Nickase Plasmid (h) and WDR82 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WDR82. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WDR82 Antibody (A-2): sc-518243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR82 Double Nickase Plasmid (h) | sc-406963-NIC | 20 µg | $410.00 | |||
WDR82 Double Nickase Plasmid (h2) | sc-406963-NIC-2 | 20 µg | $410.00 |
Human WDR82 encodes a WD repeat–containing protein that functions as a regulatory adaptor in nuclear complexes controlling transcription and chromatin state. WDR82 is best known for coupling RNA polymerase II C-terminal domain phosphorylation status to recruitment of histone-modifying activities, including H3K4 methylation machinery, thereby influencing promoter-proximal gene regulation. Through these roles, it contributes to RNA processing and transcriptional fidelity in pathways that coordinate gene expression programs across cell cycle and differentiation contexts. Dysregulation of WDR82-associated chromatin and transcriptional control has been examined in cancer biology and other disorders linked to aberrant epigenetic regulation.
WDR82 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WDR82 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WDR82. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WDR82 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WDR82-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.