Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
WDR77 Double Nickase Plasmid (h): sc-403890-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WDR77 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WDR77 Double Nickase Plasmid (h) and WDR77 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting WDR77. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: WDR77 Antibody (C-2): sc-376549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR77 Double Nickase Plasmid (h) | sc-403890-NIC | 20 µg | $410.00 | |||
WDR77 Double Nickase Plasmid (h2) | sc-403890-NIC-2 | 20 µg | $410.00 |
WDR77 (also known as MEP50) encodes a WD-repeat scaffolding protein that serves as an obligate cofactor for PRMT5, supporting symmetric dimethylation of arginine residues on histones and diverse RNA-binding proteins. Through this PRMT5–WDR77 methylosome complex, WDR77 influences chromatin organization, transcriptional control, and pre-mRNA splicing, with downstream effects on cell-cycle progression and lineage programs. WDR77 has also been linked to androgen receptor co-regulation and broader nuclear receptor signaling, connecting it to pathways that shape proliferation and differentiation states. Dysregulated PRMT5/WDR77 activity is frequently studied in cancer biology and developmental contexts where epigenetic and RNA-processing defects contribute to altered gene expression networks.
WDR77 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the WDR77 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within WDR77. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt WDR77 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of WDR77-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.