Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VPRBP Double Nickase Plasmid (h): sc-403283-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VPRBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VPRBP Double Nickase Plasmid (h) and VPRBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DCAF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VPRBP Antibody (C-8): sc-376850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPRBP Double Nickase Plasmid (h) | sc-403283-NIC | 20 µg | $410.00 | |||
VPRBP Double Nickase Plasmid (h2) | sc-403283-NIC-2 | 20 µg | $410.00 |
DCAF1 encodes VPRBP, a substrate receptor for the CUL4–DDB1 E3 ubiquitin ligase complex that helps direct ubiquitination and proteasomal turnover of specific protein targets. Through regulation of protein stability, VPRBP contributes to control of cell-cycle progression, DNA damage responses, and chromatin-associated processes that influence transcriptional programs. VPRBP has also been linked to signaling networks involving kinase regulation and checkpoint control, making it relevant to studies of genome integrity and proliferative stress. Dysregulation of CUL4–DDB1–DCAF1 activity has been associated with altered proteostasis in cancer-related contexts and other disorders where ubiquitin-mediated regulation is disrupted.
VPRBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DCAF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DCAF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DCAF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DCAF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.